Navikas V, Link J, Palasik W, Söderström M, Fredrikson S, Olsson T, Link H
Department of Neurology and Ophthalmology, Karolinska Institute, Huddinge Hospital, Stockholm, Sweden.
Scand J Immunol. 1995 Feb;41(2):171-8. doi: 10.1111/j.1365-3083.1995.tb03550.x.
The inflammatory nature of multiple sclerosis (MS) implicates the participation of immunoregulatory cytokines, including the Th2 related IL-10. We describe the use of in situ hybridization with cDNA oligonucleotide probes to detect and enumerate mononuclear cells (MNC) expressing mRNA for IL-10, which is known to down-regulate Th1 cell related cytokines such as interferon-gamma. Expression of IL-10 was studied in blood MNC of MS and blood and cerebrospinal fluid (CSF) MNC of optic neuritis (ON) patients without culture and after culture in the presence of myelin basic protein (MBP), the control antigen acetylcholine receptor (AChR), and without antigen. Numbers of IL-10 mRNA expressing MNC were elevated in the MS patients' blood both when enumerated without culture and after culture with MBP. Control patients with myasthenia gravis had elevated numbers of AChR-reactive IL-10 mRNA expressing cells, while numbers of MBP-reactive IL-10 positive cells did not differ from numbers registered in cells without antigen. Patients with ON, in many instances representing early MS, had IL-10 positive blood MNC that were elevated to the same extent as in clinically definite MS, and further increased in the CSF. ON patients examined within 1 month after onset had lower numbers of MBP induced IL-10 mRNA expressing blood MNC compared with patients examined later suggesting that IL-10 is related to the degree of inflammation and outcome in ON.
多发性硬化症（MS）的炎症性质表明免疫调节细胞因子参与其中，包括与Th2相关的白细胞介素-10（IL-10）。我们描述了使用cDNA寡核苷酸探针进行原位杂交，以检测和计数表达IL-10 mRNA的单核细胞（MNC），已知IL-10可下调Th1细胞相关的细胞因子，如干扰素-γ。在未经培养以及在髓鞘碱性蛋白（MBP）、对照抗原乙酰胆碱受体（AChR）存在下培养和无抗原培养后，研究了MS患者血液MNC以及视神经炎（ON）患者血液和脑脊液（CSF）MNC中IL-10的表达。在未经培养计数时以及与MBP共培养后，MS患者血液中表达IL-10 mRNA的MNC数量均升高。重症肌无力对照患者中表达AChR反应性IL-10 mRNA的细胞数量增加，而MBP反应性IL-10阳性细胞数量与无抗原培养的细胞中记录的数量无差异。在许多情况下代表早期MS的ON患者，其血液中IL-10阳性MNC升高至与临床确诊MS相同的程度，并且在CSF中进一步增加。与后期检查的患者相比，发病后1个月内检查的ON患者中MBP诱导的表达IL-10 mRNA的血液MNC数量较低，这表明IL-10与ON中的炎症程度和预后相关。
