内质网Ca(2+) -ATP酶抑制剂2,5 -二（叔丁基）-1,4 -对苯二酚对激动剂刺激的子宫肌层相性收缩的影响

Effects of 2,5-di(tert-butyl)-1,4-hydroquinone, an endoplasmic reticulum Ca(2+)-ATPase inhibitor, on agonist-stimulated phasic myometrial contractions.

作者信息

Phillippe M, Kim J, Freij M, Saunders T

机构信息

Department of Obstetrics & Gynecology, University of Chicago, Illinois 60637.

出版信息

Biochem Biophys Res Commun. 1995 Feb 27;207(3):891-6. doi: 10.1006/bbrc.1995.1269.

DOI:10.1006/bbrc.1995.1269

PMID:7532406

Abstract

Phasic myometrial contractions utilize mechanisms involving the cycling of calcium into and out of intracellular calcium stores. These studies were performed to determine the effects of 2,5-di(tert-butyl)-1,4-hydroquinone (tBHQ), an endoplasmic reticulum Ca(2+)-ATPase inhibitor, on in vitro isometric myometrial contractions. These studies demonstrated that low concentrations of tBHQ (eg. 10 microM) appear to inhibit intracellular calcium cycling, whereas higher concentrations also inhibit extracellular calcium influx. These combined tBHQ effects markedly suppressed myometrial contractions stimulated in response to various agonists including oxytocin, PGF2 alpha, KCl, ionomycin, and Bay K 8644.

摘要

子宫肌层的阶段性收缩利用了涉及钙离子进出细胞内钙库循环的机制。进行这些研究以确定内质网Ca(2+)-ATP酶抑制剂2,5-二（叔丁基）-1,4-对苯二酚（tBHQ）对体外子宫肌层等长收缩的影响。这些研究表明，低浓度的tBHQ（例如10微摩尔）似乎抑制细胞内钙循环，而较高浓度也抑制细胞外钙内流。tBHQ的这些联合作用显著抑制了对包括催产素、前列腺素F2α、氯化钾、离子霉素和Bay K 8644在内的各种激动剂产生反应而刺激的子宫肌层收缩。