Hecker M, Nematollahi H, Hey C, Busse R, Racké K
Center of Physiology, Johann Wolfgang Goethe University Clinic, Frankfurt am Main, Germany.
FEBS Lett. 1995 Feb 13;359(2-3):251-4. doi: 10.1016/0014-5793(95)00039-c.
The hypothesis was investigated that the nitric oxide (NO) synthase intermediate, NG-hydroxy-L-arginine (HOArg), is an arginase inhibitor in rabbit or rat alveolar macrophages. Exogenously applied HOArg strongly inhibited the arginase activity present in these cells (IC50 > or = 15 microM), and attenuated L-[3H]arginine transport (IC50 > or = 500 microM) in rabbit alveolar macrophages. Moreover, up to 37 microM HOArg were detected in the conditioned medium, but not in the lysate, of rat alveolar macrophages exposed to bacterial lipopolysaccharide for 18 h. HOArg may thus be a potent endogenous arginase inhibitor in these cells which increases the availability of L-arginine for NO biosynthesis.
研究了一氧化氮（NO）合酶中间体NG-羟基-L-精氨酸（HOArg）是兔或大鼠肺泡巨噬细胞中精氨酸酶抑制剂的假说。外源性应用HOArg可强烈抑制这些细胞中的精氨酸酶活性（IC50≥15μM），并减弱兔肺泡巨噬细胞中L-[3H]精氨酸的转运（IC50≥500μM）。此外，在暴露于细菌脂多糖18小时的大鼠肺泡巨噬细胞的条件培养基中检测到高达37μM的HOArg，但在裂解物中未检测到。因此，HOArg可能是这些细胞中一种有效的内源性精氨酸酶抑制剂，可增加L-精氨酸用于NO生物合成的可用性。
