Kasper M, Sakai K, Koslowski R, Wenzel K W, Haroske G, Schuh D, Müller M
Institute of Pathology, Technical University of Dresden, Germany.
Histochemistry. 1994 Nov;102(5):345-52. doi: 10.1007/BF00268905.
The colocalization of surfactant protein A (SP-A) and the alveolar macrophage markers ED1 and RM-1, as well as various lectins of the N-acetyl-galactosamine group [Maclura pomifera lectin (MPA), Dolichos biflorus lectin (DBA), soybean agglutinin (SBA)] and of the mannose group [Canavalia ensiformis lectin (ConA), Galanthus nivalis lectin (GNA)] was studied in normal and fibrotic rat lung tissues. In normal tissue, SP-A was located preferentially in the alveolar macrophage subpopulation lacking specific binding sites for lectins of the N-acetylgalactosamine group (DBA and SBA), although 50% of MPA-binding macrophages contained SP-A. The ED1-positive cells were SP-A-negative, whereas SP-A uptake could be detected among the RM-1 immunoreactive as well as the ConA and GNA binding macrophages. In fibrotic lung tissue, however, a small number of DBA and SBA binding macrophages contained SP-A and the percentage of GNA and ConA binding alveolar macrophages exhibiting SP-A immunoreactivity was reduced. Additionally, the number of ED1+/SP-A+ macrophages was found to be increased. Immunoelectron microscopy revealed accumulation of SP-A in the extracellular space. The differing SP-A content in different alveolar macrophage subpopulations suggests a more complex mechanism of uptake and degradation of surfactant proteins in normal and pathological conditions, which cannot simply be explained by the glycoconjugate pattern on the surface of alveolar macrophages.
在正常和纤维化大鼠肺组织中，研究了表面活性蛋白A（SP-A）与肺泡巨噬细胞标志物ED1和RM-1，以及N-乙酰半乳糖胺基团的各种凝集素[桑科榕属植物凝集素（MPA）、双花扁豆凝集素（DBA）、大豆凝集素（SBA）]和甘露糖基团的凝集素[刀豆凝集素（ConA）、雪花莲凝集素（GNA）]的共定位情况。在正常组织中，SP-A优先位于缺乏N-乙酰半乳糖胺基团凝集素（DBA和SBA）特异性结合位点的肺泡巨噬细胞亚群中，尽管50%与MPA结合的巨噬细胞含有SP-A。ED1阳性细胞为SP-A阴性，而在RM-1免疫反应性以及ConA和GNA结合的巨噬细胞中可检测到SP-A摄取。然而，在纤维化肺组织中，少数与DBA和SBA结合的巨噬细胞含有SP-A，且表现出SP-A免疫反应性的GNA和ConA结合肺泡巨噬细胞的百分比降低。此外，发现ED1+/SP-A+巨噬细胞的数量增加。免疫电子显微镜显示SP-A在细胞外空间积聚。不同肺泡巨噬细胞亚群中SP-A含量的差异表明，在正常和病理条件下，表面活性蛋白的摄取和降解机制更为复杂，不能简单地用肺泡巨噬细胞表面的糖缀合物模式来解释。
