Pérez-Arellano J L, Losa-García J E, Orfao-Matos A, González M, de la Cruz J L, Jiménez A, de Castro S
Departamento de Medicina, Universidad de Salamanca, Spain.
Diagn Cytopathol. 1993;9(3):259-65. doi: 10.1002/dc.2840090304.
The alveolar macrophage (AM) plays an essential role in the pathogenesis of interstitial lung diseases. The expression of specific membrane antigens is related to the functional or madurative status of the cells of mononuclear phagocyte system. The aim of this study was to analyze the expression of several markers (HLA-DR, CD11b, CD16, CD14) in AM obtained by bronchoalveolar lavage from control patients (n = 6), patients with sarcoidosis (n = 6), diffuse neoplastic infiltration of the lung (n = 7), pulmonary fibrosis (n = 4), and hypersensitivity pneumonitis (n = 3) by two evaluation techniques (flow cytometry and alkaline immunophosphatase). In the light of the results we can conclude that in the immunophenotypical study of the alveolar macrophage, flow cytometry (with semiquantitative evaluation to avoid the problem of autofluorescence) is a useful tool in the evaluation of those antigens that are only weakly or moderately expressed on AM (CD11b or CD14), whereas the alkaline immunophosphatase technique is of great interest in the evaluation of those that are strongly expressed (i.e., HLA DR). Additionally, the variable expression of the different antigens in the different alveolar-interstitial pathological states is patent in some diseases.
肺泡巨噬细胞（AM）在间质性肺疾病的发病机制中起重要作用。特定膜抗原的表达与单核吞噬细胞系统细胞的功能或成熟状态有关。本研究的目的是通过两种评估技术（流式细胞术和碱性免疫磷酸酶法）分析从对照患者（n = 6）、结节病患者（n = 6）、肺弥漫性肿瘤浸润患者（n = 7）、肺纤维化患者（n = 4）和过敏性肺炎患者（n = 3）的支气管肺泡灌洗中获得的肺泡巨噬细胞中几种标志物（HLA-DR、CD11b、CD16、CD14）的表达。根据结果我们可以得出结论，在肺泡巨噬细胞的免疫表型研究中，流式细胞术（采用半定量评估以避免自发荧光问题）是评估在肺泡巨噬细胞上仅弱表达或中等表达的抗原（CD11b或CD14）的有用工具，而碱性免疫磷酸酶技术在评估强表达的抗原（即HLA DR）方面具有重要意义。此外，在某些疾病中，不同肺泡 - 间质病理状态下不同抗原的可变表达是明显的。
