Hitotsumatsu T, Iwaki T, Fukui M, Tateishi J
Department of Neuropathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Acta Neuropathol. 1994;88(6):501-10. doi: 10.1007/BF00296486.
Round granulated body (RGB) and eosinophilic hyaline droplets (EHDs) have been described as cytoplasmic inclusions of certain astrocytic tumors. In the previous literature, however, these inclusions have been described using various terms or regarded as nosologically the same entity. Light microscopically, RGB appeared as a round discrete body filled with fine uniform granules, while EHDs demonstrated a cluster of bright eosinophilic, round objects of various size. They could be clearly distinguished even by conventional histochemical staining such as the Masson trichrome stain and the phosphotungstic acid hematoxylin preparation. Both RGB and EHDs expressed positive immunoreactions for glial fibrillary acidic protein, several lysosomal markers, and some stress-response proteins. The ultrastructural appearances of these inclusions were distinct, however, one common feature was that they consisted of aggregations of numerous membrane-bound electron-dense bodies. Thus, both inclusions appear to be produced by neoplastic astrocytes and are possibly related to the lysosomal system. We examined the presence of RGB and EHDs in 138 astrocytic tumors. Both inclusions occurred most frequently in pleomorphic xanthoastrocytomas, followed by gangliogliomas and pilocytic astrocytomas. Subependymal giant cell astrocytomas exhibited only RGBs. RGBs and EHDs were not seen in any abundance in glioblastomas, gliosarcomas, fibrillary astrocytomas, protoplasmic astrocytomas, or oligo-astrocytomas. Some glioblastomas, however, showed only EHDs in small numbers. Several anaplastic astrocytomas were associated with a large number of RGBs and/or EHDs, and they revealed only rare mitosis despite marked cellular pleomorphism. Although RGB and EHDs have different morphological features, the presence of these inclusions in abundance may represent either a degenerative change, a long-standing lesion, or an indolent growth of the astrocytic tumors.
圆形颗粒体（RGB）和嗜酸性透明滴（EHDs）已被描述为某些星形细胞瘤的细胞质内含物。然而，在以往的文献中，这些内含物使用了各种术语进行描述，或者在分类学上被视为同一实体。在光学显微镜下，RGB表现为一个圆形离散体，充满细小均匀的颗粒，而EHDs则显示为一簇明亮的嗜酸性圆形物体，大小各异。即使通过传统的组织化学染色，如Masson三色染色和磷钨酸苏木精制片，它们也能被清楚地区分。RGB和EHDs对胶质纤维酸性蛋白、几种溶酶体标记物和一些应激反应蛋白均呈阳性免疫反应。然而，这些内含物的超微结构外观是不同的，一个共同特征是它们由大量膜结合电子致密体的聚集物组成。因此，这两种内含物似乎都是由肿瘤性星形胶质细胞产生的，并且可能与溶酶体系统有关。我们检查了138例星形细胞瘤中RGB和EHDs的存在情况。这两种内含物最常见于多形性黄色星形细胞瘤，其次是节细胞胶质瘤和毛细胞型星形细胞瘤。室管膜下巨细胞星形细胞瘤仅表现为RGBs。在胶质母细胞瘤、胶质肉瘤、纤维型星形细胞瘤、原浆型星形细胞瘤或少突-星形细胞瘤中，RGBs和EHDs均未大量出现。然而，一些胶质母细胞瘤仅少量显示EHDs。一些间变性星形细胞瘤与大量的RGBs和/或EHDs相关，尽管细胞明显多形性，但它们仅显示罕见的有丝分裂。尽管RGB和EHDs具有不同的形态特征，但这些内含物大量存在可能代表星形细胞瘤的退行性改变、长期病变或惰性生长。
