Liberski P P
Department of Molecular Biology, School of Medicine, Lódź.
Folia Neuropathol. 1998;36(3):161-77.
In order to complete the classical descriptions of astrocytic tumors, I report here ultrastructural findings regarding this group of neoplasms. Fibrillary astrocytomas consisted of a mixture of cell bodies and their processes. Nuclei were indented and contained various types of nuclear bodies or filamentous paracrystalline inclusions. The cytoplasm with innumerable glial filaments was observed. Gemistocytic cells contained slightly more indented peripherally located nuclei while the whole cytoplasm was densely filled with glial filaments. The typical cells for the granular part of the astrocytic tumors and the granular tumors of astrocytic lineage contained the intracytoplasmic bundles of glial filaments and membrane-bound autophagic vacuoles. Pilocytic astrocytes were in the typical way elongated and contained many glial filaments in parallel bundles. Hirano bodies were rarely seen. Rosenthal fibers consisted of large electron-dense granular or amorphous masses merging at the periphery with glial filaments. In a case of the pleomorphic xanthoastrocytoma the electron microscopy showed numerous astrocytes connected by many long plaque-like junctions or hemidesmosomes with dense bundles of collagen fibrils. The majority of the astrocytes were covered with basal laminae, forming a complicated labyrinth-like pattern invaginated in astrocytic cytoplasm. Other astrocytes included also numerous vacuoles containing myelin figures and whorls. In a case of the subependymal giant cell astrocytoma, giant cells contained innumerable glial filaments, numerous ribosomes, distended endoplasmic reticulum, electron-dense bodies and peculiar crystalloids. Secondary glioblastomas retained clear stigmata of astrocytic tumor but the nuclei seemed to be more intended, cisterns of the endoplasmic reticulum appeared to be distended and frequently intranuclear pseudoinclusions were observed.
为了完善星形细胞瘤的经典描述，我在此报告关于这组肿瘤的超微结构研究结果。纤维性星形细胞瘤由细胞体及其突起混合组成。细胞核呈凹陷状，含有各种类型的核体或丝状副晶状包涵体。观察到细胞质中有无数的胶质丝。肥胖型星形细胞的细胞核周边略凹陷，整个细胞质充满密集的胶质丝。星形细胞瘤颗粒部分及星形细胞系颗粒细胞瘤的典型细胞含有胞质内的胶质丝束和膜结合自噬泡。毛细胞型星形胶质细胞呈典型的细长形，含有许多平行排列的胶质丝。很少见到 Hirano 小体。Rosenthal 纤维由大的电子致密颗粒状或无定形团块组成，在周边与胶质丝融合。在一例多形性黄色星形细胞瘤中，电镜显示许多星形细胞通过许多长的斑块状连接或半桥粒与密集的胶原纤维束相连。大多数星形细胞被基膜覆盖，形成一个复杂的迷宫样模式，向星形细胞胞质内凹陷。其他星形细胞还包括许多含有髓鞘样结构和涡旋的空泡。在一例室管膜下巨细胞星形细胞瘤中，巨细胞含有无数的胶质丝、大量核糖体、扩张的内质网、电子致密体和特殊的晶体。继发性胶质母细胞瘤保留了明显的星形细胞肿瘤特征，但细胞核似乎更不规则，内质网池似乎扩张，且经常观察到核内假包涵体。
