Matyas J R, Adams M E, Huang D, Sandell L J
University of Calgary Health Sciences Centre, Alberta, Canada.
Arthritis Rheum. 1995 Mar;38(3):420-5. doi: 10.1002/art.1780380320.
To quantify the gene expression of aggrecan core protein and type II collagen in an experimental animal model of osteoarthritis (OA).
Total RNA was extracted from the articular cartilage of unoperated knee joints and from OA joints produced by anterior cruciate ligament transection. The relative amounts of type II collagen and aggrecan core protein messenger RNA (mRNA) were evaluated by Northern blot analysis.
Total RNA was elevated 2.5 times, aggrecan mRNA was elevated 2 times, and type II collagen mRNA was elevated 8 times, in OA knees compared to unoperated controls.
Chondrocytes are activated metabolically in response to joint injury. Discoordinate gene expression of aggrecan and type II collagen is characteristic of early experimental OA, and we speculate that it may contribute to its pathogenesis.
在骨关节炎（OA）实验动物模型中对聚集蛋白聚糖核心蛋白和II型胶原蛋白的基因表达进行定量分析。
从未手术膝关节的关节软骨以及前交叉韧带横断所致OA关节中提取总RNA。通过Northern印迹分析评估II型胶原蛋白和聚集蛋白聚糖核心蛋白信使核糖核酸（mRNA）的相对含量。
与未手术对照组相比，OA膝关节中总RNA升高2.5倍，聚集蛋白聚糖mRNA升高2倍，II型胶原蛋白mRNA升高8倍。
软骨细胞在关节损伤时发生代谢激活。聚集蛋白聚糖和II型胶原蛋白的不协调基因表达是早期实验性OA的特征，我们推测这可能与其发病机制有关。
