Chaubey M, Singhal S S, Awasthi S, Saxena M, Dyer R B, Awasthi Y C, Herzog N K
Department of Human Biological Chemistry, University of Texas Medical Branch, Galveston 77555.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1994 Jul;108(3):311-9.
The basal levels of mu and pi class glutathione S-transferases RNA were 18-fold higher in the male mouse liver as compared with the female. When 0.75% (w/w) BHA was included in the diet it altered the RNA levels of alpha, mu, pi GST classes and mGSTA4-4 in a tissue and sex specific manner. The most marked induction of RNA was seen for the mu class GSTs of female liver, lung and kidney (52, 10 and 8-fold, respectively), and of male liver and kidney (25 and 3.5-fold, respectively), the pi class GSTs of female liver, lung, and kidney (11, 10, and 5-fold, respectively), and mGSTA4-4 of female liver (4-fold). The effect of BHA on the induction of the mu and pi class GST RNA was 2-9 fold greater in female as compared with male tissues. The degree of induction of GST RNA did not correlate directly with changes in GST protein indicating that post-transcriptional events regulating GST expression may be affected by BHA particularly for GST mu and mGSTA4-4.
雄性小鼠肝脏中μ和π类谷胱甘肽S-转移酶RNA的基础水平比雌性高18倍。当饮食中含有0.75%（w/w）的丁基羟基茴香醚（BHA）时，它会以组织和性别特异性的方式改变α、μ、π类谷胱甘肽S-转移酶（GST）以及mGSTA4-4的RNA水平。RNA诱导最为明显的是雌性肝脏、肺和肾脏中的μ类谷胱甘肽S-转移酶（分别为52倍、10倍和8倍），以及雄性肝脏和肾脏中的μ类谷胱甘肽S-转移酶（分别为25倍和3.5倍），雌性肝脏、肺和肾脏中的π类谷胱甘肽S-转移酶（分别为11倍、10倍和5倍），以及雌性肝脏中的mGSTA4-4（4倍）。与雄性组织相比，BHA对μ和π类谷胱甘肽S-转移酶RNA诱导的作用在雌性中要大2至9倍。谷胱甘肽S-转移酶RNA的诱导程度与谷胱甘肽S-转移酶蛋白的变化没有直接相关性，这表明调节谷胱甘肽S-转移酶表达的转录后事件可能受到BHA的影响，尤其是对于谷胱甘肽S-转移酶μ和mGSTA4-4。
