Porteros A, Alonso J R, Arévalo R, García-Ojeda E, Crespo C, Aijón J
Departamento de Biología Celular y Patología, Universidad de Salamanca, Spain.
Chem Senses. 1994 Oct;19(5):413-24. doi: 10.1093/chemse/19.5.413.
The distribution of NADPH-diaphroase activity was examined in the accessory olfactory bulb of the rat using a direct histochemical technique. Labeled fibers and somata were found in all layers of the accessory olfactory bulb. The entire vomeronasal nerve and all vomeronasal glomeruli were strongly labeled, contrary to the main olfactory bulb, where only dorsomedial olfactory glomeruli displayed NADPH-diaphorase activity. NADPH-diaphorase positive neurons were identified as periglomerular cells in the glomerular layer and external plexiform layer, horizontal cells in the internal plexiform layer, and granule cells and deep short-axon cells in the granule cell layer. The labeled dendrites of the granule cells formed a dense neuropile in the granule cell layer, internal plexiform layer and external plexiform layer. The staining pattern in the accessory olfactory bulb was more complex than what has been previously reported, and demonstrated both similarities and differences with the distribution of NADPH-diaphorase in the main olfactory bulb.
采用直接组织化学技术，对大鼠副嗅球中还原型辅酶Ⅱ-黄递酶（NADPH-diaphorase）活性的分布进行了研究。在副嗅球的所有层中均发现了标记的纤维和细胞体。与主嗅球相反，整个犁鼻神经和所有犁鼻小体均被强烈标记，在主嗅球中只有背内侧嗅小体显示出NADPH-黄递酶活性。NADPH-黄递酶阳性神经元在小球层和外丛状层中被鉴定为小球周围细胞，在内丛状层中为水平细胞，在颗粒细胞层中为颗粒细胞和深短轴突细胞。颗粒细胞的标记树突在颗粒细胞层、内丛状层和外丛状层中形成了密集的神经毡。副嗅球中的染色模式比先前报道的更为复杂，并且与主嗅球中NADPH-黄递酶的分布既有相似之处又有差异。
