Scott J W, McDonald J K, Pemberton J L
J Comp Neurol. 1987 Jun 15;260(3):378-91. doi: 10.1002/cne.902600305.
Several types of short axon cells of the mammalian olfactory bulb have been described after Golgi impregnation. Two of these types have been observed in our material after treatment with the NADPH-diaphorase procedure or after immunohistochemistry for neuropeptide-Y (NPY). The cells stained by the two procedures have similar morphologies and distributions. A less extensive series of observations confirms that similar cells also display somatostatin (SS)-like immunoreactivity. One of these cell types corresponds to the superficial short axon cell of Golgi and electron microscopic studies. The dendrites of this cell lie within the periglomerular region and in the superficial external plexiform layer (EPL), generally lying parallel to the glomerular layer. In some cases the axon has been traced across the EPL into the granule cell layer (GCL). This cell may provide another route of interaction between the periglomerular region and the granule cells in addition to the influences conducted by basal dendrites and axon collaterals of some mitral and tufted cells. A type of deep short axon cell is also visible with these two procedures. It lies deep in the granule cell layer, frequently near the ventricular layer and its dendrites lie parallel to that layer. This deep short axon cell is stained with much greater frequency by the NADPH-diaphorase and NPY procedures than is the superficial short axon cell. It corresponds most closely to the Blanes or Golgi cells of the Golgi impregnation literature, but it appears to differ from these cells in the position and orientation of its dendrites. No spines have been observed on either the superficial or deep cells in this series. Many glomeruli are also stained by the NADPH-diaphorase procedure, but are not NPY or SS immunoreactive. This may provide additional evidence for functional differences between glomeruli in local regions of the olfactory bulb.
经高尔基染色法浸染后，已对哺乳动物嗅球中的几种短轴突细胞类型进行了描述。在用还原型辅酶Ⅱ黄递酶法处理后或进行神经肽Y（NPY）免疫组织化学检测后，我们在材料中观察到了其中两种类型。这两种方法染色的细胞具有相似的形态和分布。一系列不太广泛的观察结果证实，类似的细胞也显示出促生长激素抑制素（SS）样免疫反应性。这些细胞类型之一与高尔基染色法及电子显微镜研究中的浅层短轴突细胞相对应。该细胞的树突位于小球周围区域和浅层外丛状层（EPL）内，通常与小球层平行。在某些情况下，轴突已被追踪到穿过EPL进入颗粒细胞层（GCL）。除了一些二尖瓣细胞和簇状细胞的基底树突和轴突侧支传导的影响外，这种细胞可能为小球周围区域和颗粒细胞之间提供了另一种相互作用途径。用这两种方法还可以看到一种深层短轴突细胞。它位于颗粒细胞层深处，经常靠近脑室层，其树突与该层平行。与浅层短轴突细胞相比，这种深层短轴突细胞经还原型辅酶Ⅱ黄递酶法和NPY法染色的频率要高得多。它与高尔基染色文献中的布拉内斯细胞或高尔基细胞最为接近，但在树突的位置和方向上似乎与这些细胞不同。在该系列的浅层或深层细胞上均未观察到棘突。许多小球也经还原型辅酶Ⅱ黄递酶法染色，但无NPY或SS免疫反应性。这可能为嗅球局部区域中小球之间的功能差异提供额外证据。
