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不同身体部位的重金属含量(Hg2+、Cd2+、Pb2+):其对成年牧草蝗胆碱酯酶活性和结合糖蛋白的影响。

Heavy metal content (Hg2+, Cd2+, Pb2+) in various body parts: its impact on cholinesterase activity and binding glycoproteins in the grasshopper Aiolopus thalassinus adults.

作者信息

Schmidt G H, Ibrahim N M

机构信息

Department of Zoology-Entomology, University of Hannover, Federal Republic of Germany.

出版信息

Ecotoxicol Environ Saf. 1994 Nov;29(2):148-64. doi: 10.1016/0147-6513(94)90016-7.

DOI:10.1016/0147-6513(94)90016-7
PMID:7533707
Abstract

Various toxicological symptoms were observed in Aiolopus thalassinus (Fabr.) adults which resulted from either contaminated soil or from being fed HgCl2-, CdCl2-, and PbCl2-treated diet. Many insects had abnormal wings which were present until the F3 untreated generation. Heavy metals were stored in different body parts, most of Cd2+ and Hg2+ were found in the testes, followed by the gut. Lead was enriched in all organs and body parts of the treated grasshoppers compared to the control. The highest lead concentrations were found in testes, wings, gut, and ovaries. In the treated generation ChE activity was reduced to about 23% compared to the untreated grasshoppers. In contrast to this, lead increased ChE activity to about 14%. In the following F2 untreated adults, ChE activity was normal; no long-term effect was found. In the supernatant of treated adults a Cd-type glycoprotein was found which can bind Cd ions; its MW was determined by electrophoresis and reference proteins at about 14,450 Da.

摘要

在因土壤污染或食用经HgCl2、CdCl2和PbCl2处理的饲料而受到影响的成年黄胫小车蝗(Aiolopus thalassinus (Fabr.))中观察到了各种毒理学症状。许多昆虫的翅膀出现异常,这种情况一直持续到第三代未处理的后代。重金属储存在不同的身体部位,大部分Cd2+和Hg2+存在于睾丸中,其次是肠道。与对照组相比,处理后的蝗虫体内所有器官和身体部位的铅含量都有所增加。睾丸、翅膀、肠道和卵巢中的铅浓度最高。在处理后的这一代中,与未处理的蝗虫相比,胆碱酯酶(ChE)活性降低至约23%。与此相反,铅使ChE活性增加至约14%。在接下来的未处理的F2代成虫中,ChE活性正常;未发现长期影响。在处理后的成虫的上清液中发现了一种能结合Cd离子的Cd型糖蛋白;其分子量通过电泳和参考蛋白测定约为14450道尔顿。

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