Würdig S, Wolf G
Institute of Medical Neurobiology, Medical Faculty, University of Magdeburg, Germany.
J Hirnforsch. 1994;35(4):495-9.
The activity of reduced nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) was examined histochemically in the rat cerebellar cortex at the light and electron microscopical level. Different staining patterns were observed depending on the technique used. Electron dense reaction product was seen on distinct membrane portions of the endoplasmic reticulum including the nuclear envelope in most of the neurons and in endothelial cells. Electron microscopically no activity staining was seen in glial cells, including Bergmann cells. The addition of the detergent Triton X-100, usually applied in the light microscopical diaphorase histochemistry, led to a striking diminution in membrane staining. In such preparations inspected electron microscopically formazan formed small granules which were evenly distributed over the cytoplasm.
在光镜和电镜水平上，采用组织化学方法检测了大鼠小脑皮质中还原型烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）的活性。根据所使用的技术，观察到了不同的染色模式。在大多数神经元和内皮细胞中，在内质网的不同膜部分包括核膜上可见电子致密反应产物。在电镜下，包括伯格曼细胞在内的神经胶质细胞未见活性染色。通常用于光镜黄递酶组织化学的去污剂Triton X-100的加入导致膜染色显著减少。在电镜检查的此类标本中，甲臜形成小颗粒，均匀分布于细胞质中。
