Liu H P, Leong S K, Tay S S
Department of Anatomy, Faculty of Medicine, National University of Singapore, Kent Ridge Crescent.
J Hirnforsch. 1994;35(4):501-10.
In the present study, nicotinamide adenine dinucleotide hydrogen phosphate-diaphorase (NADPH-d) histochemistry has been used as a marker for nitric oxide synthase. NADPH-d positive neuronal cell bodies and fibres have been localized in the pancreas of the mouse, rat, chick, kitten and monkey. In all these species, most of the NADPH-d labelled neuronal cell bodies in the ganglia were found in the interlobular and interacinar connective tissue while some were intimately associated with pancreatic ducts and blood vessels. There was, however, a gradation of innervation amongst the species. Only in the pancreatic islets of the mouse and monkey were NADPH-d positive neuronal cell bodies and nerve fibres detected in the formation of neuro-insular complexes. Besides labelling of neuronal cell bodies, nerve fibres and endothelial cells, the islet cells of the mouse, kitten and chick pancreas also gave a light positive reaction for NADPH-d activity. The nerve fibre and the outer core of the connective tissue of the Pacinian corpuscles in the kitten pancreas were also labelled for NADPH-d activity. It is concluded that nitric oxide may play an important role in the neural regulation of both exocrine and endocrine secretion and in controlling the activity of the blood vessels and ducts of the pancreas.
在本研究中，烟酰胺腺嘌呤二核苷酸磷酸 - 黄递酶（NADPH-d）组织化学已被用作一氧化氮合酶的标志物。NADPH-d阳性神经元细胞体和纤维已定位在小鼠、大鼠、鸡、小猫和猴子的胰腺中。在所有这些物种中，神经节中大多数NADPH-d标记的神经元细胞体位于小叶间和腺泡间结缔组织中，而有些则与胰管和血管密切相关。然而，不同物种之间的神经支配存在程度差异。仅在小鼠和猴子的胰岛中，在神经胰岛复合体的形成过程中检测到NADPH-d阳性神经元细胞体和神经纤维。除了标记神经元细胞体、神经纤维和内皮细胞外，小鼠、小猫和鸡胰腺的胰岛细胞对NADPH-d活性也呈轻度阳性反应。小猫胰腺中帕氏小体的神经纤维和结缔组织的外核也被标记为具有NADPH-d活性。结论是，一氧化氮可能在胰腺外分泌和内分泌分泌的神经调节以及控制胰腺血管和导管的活动中起重要作用。
