Vink J, Dekker S K, Van Leeuwen R L, Vermeer B J, Bruijn J A, Byers H R
Department of Dermatology and Pathology, University of Leiden, The Netherlands.
Pigment Cell Res. 1994 Oct;7(5):339-47. doi: 10.1111/j.1600-0749.1994.tb00637.x.
We characterized beta 1 integrin subunit expression on three different cultures of benign human nevomelanocytes (NMC) and on four different cell cultures of human dysplastic nevus (DN) cells by flow cytometry analysis and examined their role in mediating cell spreading and migration on collagen type IV (CN IV) and laminin (LN) coated substrates by using a quantitative video image analysis system. The seven human NMC and DNC cultures expressed heterogeneous levels of beta 1, alpha 2, alpha 3 and alpha 6 integrin subunits. Image analysis showed that a significant increase (P < 0.001) in cell spreading and migration of the DN cells was induced on increasing coating concentrations of CN IV and LN. However, the NMC did not show an increase in cell spreading or migration on these substrates when compared to the substrates coated with denatured BSA only. The CN IV-induced cell spreading of the DN cells was significantly inhibited by anti-beta 1 mAb (AIIB2), anti-alpha 2 mAb (P1E6), or anti-alpha 3 mAb (P1B5), but not by mAb against alpha 6 integrin subunit (GoH3). The DN cell spreading on LN was not significantly inhibited by these mAbs. In contrast, the migration of the DN on CN IV and LN was significantly inhibited by anti-beta 1 mAb, anti-alpha 2 mAb, anti-alpha 3 mAb and anti-alpha 6 mAb. These data suggest that the alpha 2 and alpha 3 subunit are important for cell spreading of the DN on CN IV, although they are less important in cell spreading on the extracellular matrix component LN.(ABSTRACT TRUNCATED AT 250 WORDS)
我们通过流式细胞术分析，对三种不同的良性人痣黑素细胞（NMC）培养物以及四种不同的人发育异常痣（DN）细胞培养物中的β1整合素亚基表达进行了表征，并使用定量视频图像分析系统，研究了它们在介导细胞在IV型胶原（CN IV）和层粘连蛋白（LN）包被底物上的铺展和迁移中的作用。七种人NMC和DNC培养物表达了不同水平的β1、α2、α3和α6整合素亚基。图像分析表明，随着CN IV和LN包被浓度的增加，DN细胞的铺展和迁移显著增加（P < 0.001）。然而，与仅用变性牛血清白蛋白包被的底物相比，NMC在这些底物上的细胞铺展或迁移并未增加。抗β1单克隆抗体（AIIB2）、抗α2单克隆抗体（P1E6）或抗α3单克隆抗体（P1B5）可显著抑制CN IV诱导的DN细胞铺展，但抗α6整合素亚基的单克隆抗体（GoH3）则无此作用。这些单克隆抗体对DN细胞在LN上的铺展无显著抑制作用。相反，抗β1单克隆抗体、抗α2单克隆抗体、抗α3单克隆抗体和抗α6单克隆抗体可显著抑制DN细胞在CN IV和LN上的迁移。这些数据表明，α2和α3亚基对DN细胞在CN IV上的铺展很重要，尽管它们在细胞在细胞外基质成分LN上的铺展中不太重要。（摘要截断于250字）
