Yoshinaga I G, Vink J, Dekker S K, Mihm M C, Byers H R
Department of Pathology, Harvard Medical School, Massachusetts General Hospital, Boston.
Melanoma Res. 1993 Dec;3(6):435-41. doi: 10.1097/00008390-199311000-00006.
Recent findings indicate that variable expression of beta 1 integrins may play a role in differential melanoma cell motility. Primary melanoma (PM) and metastatic melanoma (MM) cultures, derived from the same patient, were tested for their beta 1, alpha 2, alpha 3 and alpha 6 integrin subunit expression and cell migration on type IV collagen (CN IV) or laminin (LN). The MM cell line expressed markedly increased levels of the beta 1, alpha 2 and alpha 3, but not alpha 6 subunit compared to the PM cell line. The MM cell migration rate was significantly higher than that of the PM cell line on LN- or CN IV-coated substrates. Furthermore, the cell migration rate of both lines was significantly higher (p < 0.001) on these substrates than on the control substrates. The MM and PM cell migration was significantly inhibited by function-blocking anti-beta 1 and anti-alpha 3 MAbs, but not by the anti-alpha 6 MAb tested. In contrast, the anti-alpha 2 MAb significantly inhibited MM but not PM cell migration. These data show that the alpha 3 subunit plays a significant role in melanoma cell motility on CN IV and LN and that the alpha 2 subunit has a significant contribution to the motility of the MM cell line.
最近的研究结果表明，β1整合素的可变表达可能在黑色素瘤细胞的不同运动性中发挥作用。对来自同一患者的原发性黑色素瘤（PM）和转移性黑色素瘤（MM）培养物进行检测，分析其β1、α2、α3和α6整合素亚基的表达情况，以及在IV型胶原（CN IV）或层粘连蛋白（LN）上的细胞迁移能力。与PM细胞系相比，MM细胞系中β1、α2和α3亚基的表达水平显著升高，但α6亚基无明显变化。在包被有LN或CN IV的底物上，MM细胞的迁移速率显著高于PM细胞系。此外，在这些底物上，两种细胞系的迁移速率均显著高于对照底物（p < 0.001）。功能阻断性抗β1和抗α3单克隆抗体可显著抑制MM和PM细胞的迁移，但所检测的抗α6单克隆抗体无此作用。相反，抗α2单克隆抗体可显著抑制MM细胞的迁移，但对PM细胞无此作用。这些数据表明，α3亚基在黑色素瘤细胞在CN IV和LN上的运动中起重要作用，而α2亚基对MM细胞系的运动有显著贡献。
