Moreno M J, López de Pablo A L, Conde M V, Fraile M L, Marco E J
Departamento de Fisiología, Universidad Autónoma de Madrid, Spain.
Rev Esp Fisiol. 1994 Sep;50(3):175-81.
The effect of Chlorimipramine and Muscimol on serotonergic activity in rat cerebral arteries and in dorsal and median raphe nuclei were used to study the presence of a serotonergic innervation in the cerebral blood vessels functionally dependent on the brainstem nuclei activity. Serotonergic activity was appraised in rat cerebral arteries from 5-hydroxyindoleacetic acid (5-HIAA) disappearance rate or 5-hydroxytryptophan (5-HTP) accumulation after inhibiting monoamine oxidase (MAO) or aromatic amino acids decarboxylase, respectively. In dorsal and median raphe nuclei the decay with time of 5-HIAA after MAO inhibition was used to estimate serotonergic activity. Chlorimipramine significantly reduced serotonergic activity in cerebral blood vessels and in both raphe nuclei. 5-HIAA basal levels in these blood vessels were not altered by treatment with the drug. Muscimol evoked only a decrease in the serotonergic activity of the median raphe nucleus. These results suggest that rat cerebral arteries receive serotonergic fibers functionally active arising mainly from dorsal raphe nucleus.
使用氯米帕明和蝇蕈醇对大鼠脑动脉以及背侧和中缝核中5-羟色胺能活性的影响，来研究脑血管中是否存在功能上依赖于脑干核活性的5-羟色胺能神经支配。分别通过抑制单胺氧化酶（MAO）后5-羟吲哚乙酸（5-HIAA）的消失率或抑制芳香氨基酸脱羧酶后5-羟色氨酸（5-HTP）的积累，来评估大鼠脑动脉中的5-羟色胺能活性。在背侧和中缝核中，MAO抑制后5-HIAA随时间的衰减用于估计5-羟色胺能活性。氯米帕明显著降低了脑血管和两个中缝核中的5-羟色胺能活性。用该药物处理后，这些血管中的5-HIAA基础水平未发生改变。蝇蕈醇仅引起中缝核5-羟色胺能活性的降低。这些结果表明，大鼠脑动脉接受主要源自背侧中缝核的功能活跃的5-羟色胺能纤维。
