Dippel E, Haas N, Grabbe J, Schadendorf D, Hamann K, Czarnetzki B M
Department of Dermatology, University Hospital R. Virchow, Freie Universität Berlin, Germany.
Br J Dermatol. 1995 Feb;132(2):182-9. doi: 10.1111/j.1365-2133.1995.tb05011.x.
In order to investigate possible alterations in c-kit protein expression on epidermal melanocytes in different hypopigmentary disorders, we have examined skin specimens from one patient with piebaldism, one patient with naevus depigmentosus, and five patients with vitiligo. Cryosections were examined by immunohistochemistry using monoclonal antibodies against the c-kit protein (YB5.B8) and melanosomes (TA99). In piebaldism, hypomelanotic epidermis contained only a few TA99-positive epidermal melanocytes and no detectable c-kit protein, whereas in naevus depigmentosus the expression of c-kit protein was strong, and TA99 immunoreactivity was faint. In vitiligo lesions, no epidermal immunoreactivity for melanosomes or c-kit protein was found. Normally pigmented skin of all patients showed immunoreactivity of epidermal melanocytes for both c-kit protein and melanosomes. Different hypomelanotic lesions can thus be differentiated by absent melanocyte c-kit protein and low or no expression of melanosomal marker in piebaldism, normal c-kit but low melanosome expression in naevus depigmentosus, and the absence of all melanocyte markers in vitiligo.
为了研究不同色素减退性疾病中表皮黑素细胞上c-kit蛋白表达的可能变化，我们检查了1例斑驳病患者、1例色素脱失痣患者和5例白癜风患者的皮肤标本。使用抗c-kit蛋白（YB5.B8）和黑素小体（TA99）的单克隆抗体，通过免疫组织化学检查冰冻切片。在斑驳病中，色素减退的表皮仅含有少数TA99阳性的表皮黑素细胞，未检测到c-kit蛋白；而在色素脱失痣中，c-kit蛋白表达强烈，TA99免疫反应性微弱。在白癜风皮损中，未发现黑素小体或c-kit蛋白的表皮免疫反应性。所有患者的正常色素皮肤均显示表皮黑素细胞对c-kit蛋白和黑素小体均有免疫反应性。因此，不同的色素减退性皮损可通过以下方式进行区分：斑驳病中黑素细胞c-kit蛋白缺失，黑素小体标志物表达低或无表达；色素脱失痣中c-kit正常但黑素小体表达低；白癜风中所有黑素细胞标志物均缺失。
