Olsson M J, Juhlin L
Department of Dermatology, University Hospital, Uppsala, Sweden.
Br J Dermatol. 1998 Apr;138(4):644-8. doi: 10.1046/j.1365-2133.1998.02177.x.
The aim of this study was to test the usefulness of a melanocyte-enriched cell suspension for the treatment of leucoderma. After removal of a superficial (4-30 cm2) skin sample, the cells were mechanically separated in a trypsin-EDTA solution, centrifuged and washed in a melanocyte medium. The melanocyte-enriched epidermal cell suspension devoid of stratum corneum and stratum granulosum was then applied to the dermabraded depigmented skin. The 26 patients treated had piebaldism (three), vitiligo vulgaris (17), segmental vitiligo (three), halo naevi (one), naevus depigmentosus (one) and chemical leucoderma (one). In patients with widespread piebaldism we found that by diluting the cell suspension the recipient area could be increased to up to 10 times the size of the donor area with the same good results as without or with less dilution. In patients with vitiligo areas of between 50 and 90 cm2, the recipient areas were increased three- to fivefold in the donor area. Patients with piebaldism, segmental vitiligo and halo naevi healed completely, as did most patients with vitiligo. In naevus depigmentosus no effect was seen. Our new method for treatment of leucoderma has the advantage that cell culture is not needed and that it is more suitable than epidermal sheet grafts when several small areas are to be treated.
本研究的目的是测试富含黑素细胞的细胞悬液治疗白斑病的有效性。取一块浅表（4 - 30平方厘米）皮肤样本后，将细胞在胰蛋白酶 - 乙二胺四乙酸溶液中进行机械分离，离心并在黑素细胞培养基中洗涤。然后将不含角质层和颗粒层的富含黑素细胞的表皮细胞悬液应用于经磨皮处理的色素脱失皮肤。接受治疗的26例患者中，有斑驳病（3例）、寻常型白癜风（17例）、节段性白癜风（3例）、晕痣（1例）、色素脱失痣（1例）和化学性白斑病（1例）。在广泛性斑驳病患者中，我们发现通过稀释细胞悬液，受体区面积可增加至供体区面积的10倍，效果与未稀释或稀释较少时相同。在白癜风面积为50至90平方厘米的患者中，受体区面积在供体区基础上增加了三至五倍。斑驳病、节段性白癜风和晕痣患者完全治愈，大多数白癜风患者也如此。色素脱失痣患者未见效果。我们治疗白斑病的新方法具有无需细胞培养的优点，并且在需要治疗多个小面积区域时，比表皮片移植更适用。
