Nakamura S, Nishimura Y, Inagaki K, Miwa N
Department of BioTechnology, Hiroshima Prefectural University School of BioScience, Japan.
Cancer Biochem Biophys. 1994 Sep;14(2):113-21.
We showed that massive growth of mouse Ehrlich ascites carcinoma (EAC) cells was not inhibited by wool grease secreted from the sheep sebaceous gland, whereas wool fatty acids separated by saponification of wool grease was growth-inhibitory. We then fractionated wool fatty acids into 9 fractions using molecular distillation (80-200 degrees C; 1 x 10(-2) mmHg) and found a marked antitumor activity in a low-boiling-point (< 80 degrees C) fraction (MW 200-300; C10-C20), which was further separated by reversed phase liquid chromatography on an octadecylisilica gel column, resulting in 5 fractions. The second most hydrophobic fraction (C8Si-4) obtained was the most growth-inhibitory to EAC cells cultured or implanted into mice, more marked than the antitumor glycopeptide bleomycin. C8Si-4 was suggested to be a mixture of a normal-chain C16-saturated fatty acid and two branched-chain kinds of saturated C16-iso- and C19-anteiso-fatty acids without hydroxyl groups according to gas chromatography-mass spectrographic analysis. Thus low-boiling-point saturated fatty acid moieties in some wool grease molecules were shown to become growth-inhibitory in vitro and in vivo only after released in the free acid form by esterolysis.
我们发现，绵羊皮脂腺分泌的羊毛脂不会抑制小鼠艾氏腹水癌细胞（EAC）的大量生长，而通过羊毛脂皂化分离得到的羊毛脂肪酸具有生长抑制作用。然后，我们使用分子蒸馏（80 - 200℃；1×10⁻² mmHg）将羊毛脂肪酸分离成9个馏分，发现在低沸点（< 80℃）馏分（分子量200 - 300；C10 - C20）中具有显著的抗肿瘤活性，该馏分在十八烷基硅胶柱上通过反相液相色谱进一步分离，得到5个馏分。所获得的第二疏水馏分（C8Si - 4）对培养或植入小鼠体内的EAC细胞生长抑制作用最强，比抗肿瘤糖肽博来霉素更显著。根据气相色谱 - 质谱分析，C8Si - 4被认为是一种直链C16饱和脂肪酸与两种无羟基的支链饱和C16 - 异脂肪酸和C19 - 反异脂肪酸的混合物。因此，一些羊毛脂分子中的低沸点饱和脂肪酸部分只有在通过酯解以游离酸形式释放后，才在体外和体内表现出生长抑制作用。
