Henning W, Bohn W, Nebe B, Knopp A, Rychly J, Strauss M
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie, Universität Hamburg, Germany.
Eur J Cell Biol. 1994 Oct;65(1):189-99.
The expression and spatial arrangement of beta 1-integrins was determined on two immortalized liver cell lines held in coculture, namely the epithelial cell line mHepR1 and a sinusoidal endothelial cell line. On mHepR1 cells the distribution of beta 1-integrins was restricted to the basolateral plasma membrane domains, a staining pattern that is typical of polarized epithelial cells. On the endothelial cell line the beta 1-integrins were distributed all over the cell surface. In coculture the endothelial cells tended to cover over the epithelial cells. Epithelial cells located in their vicinity exhibited an increased staining of beta 1-integrins at basolateral plasma membrane domains, which was most prominent with regard to the alpha 2-subunit. When mHepR1 cells were cultivated on various types of extracellular matrix also synthesized by the endothelial cells only collagen IV was found to increase the intensity of beta 1-integrin expression at the cell surface. The results indicate that beta 1-integrin expression in epithelial cell colonies can locally be modulated by interactions with non-parenchymal cells. In addition, the data suggest that mHepR1 cells may be a favorable system for analyzing basic functions of beta 1-integrins in polarized epithelial cells.
在共培养的两种永生化肝细胞系中，即上皮细胞系mHepR1和窦状内皮细胞系，测定了β1整合素的表达和空间排列。在mHepR1细胞上，β1整合素的分布局限于基底外侧质膜结构域，这种染色模式是极化上皮细胞的典型特征。在内皮细胞系上，β1整合素分布于整个细胞表面。在共培养中，内皮细胞倾向于覆盖上皮细胞。位于其附近的上皮细胞在基底外侧质膜结构域的β1整合素染色增加，就α2亚基而言最为明显。当mHepR1细胞在各种也由内皮细胞合成的细胞外基质上培养时，仅发现IV型胶原可增加细胞表面β1整合素表达的强度。结果表明，上皮细胞集落中的β1整合素表达可通过与非实质细胞的相互作用进行局部调节。此外，数据表明mHepR1细胞可能是分析极化上皮细胞中β1整合素基本功能的良好系统。
