Farris A D, O'Brien C A, Harley J B
Oklahoma Medical Research Foundation Arthritis and Immunology Program, DVA Medical Center, Oklahoma City 73104, USA.
Gene. 1995 Mar 10;154(2):193-8. doi: 10.1016/0378-1119(94)00823-b.
YRNAs are small cytoplasmic RNAs that are components of the Ro ribonucleoprotein complex. This complex, which also includes the 60-kDa Ro protein, is a human autoantigen which is conserved among vertebrates, and is of unknown function. Multiple sequences with YRNA homology, known as YRNA-like sequences, have been detected in rabbit, mouse, duck, iguana and frog genomes with human Y cDNA probes. As judged by Northern blots of total RNA, however, not all of these genomic YRNA-like sequences are expressed. Complementary DNA and putative gene sequences for iguana Y3 (iY3) and iguana Y4 (iY4) Ro RNAs have been determined and used, along with previously sequenced human and frog Ro YRNA sequences, to construct the most likely Y3 and Y4 RNA secondary structures. The data presented indicate that Y3 is the most conserved Ro RNA, not only by its more consistent presence in other species, but also at the levels of sequence divergence and secondary structure similarity. The differences observed between the secondary structure solutions for the Y3 and Y4 Ro RNAs are consistent with the possibility that these RNAs perform different cellular functions.
YRNA是小细胞质RNA，是Ro核糖核蛋白复合体的组成成分。该复合体还包括60 kDa的Ro蛋白，是一种在脊椎动物中保守的人类自身抗原，其功能未知。用人类Y cDNA探针在兔、小鼠、鸭、鬣蜥和青蛙基因组中检测到多个与YRNA具有同源性的序列，即YRNA样序列。然而，根据总RNA的Northern印迹判断，并非所有这些基因组YRNA样序列都有表达。已确定鬣蜥Y3（iY3）和鬣蜥Y4（iY4）Ro RNA的互补DNA和推定基因序列，并将其与先前测序的人类和青蛙Ro YRNA序列一起用于构建最可能的Y3和Y4 RNA二级结构。所呈现的数据表明，Y3是最保守的Ro RNA，这不仅体现在它在其他物种中更一致的存在，还体现在序列差异和二级结构相似性水平上。Y3和Y4 Ro RNA二级结构解析结果之间的差异与这些RNA执行不同细胞功能的可能性一致。
