Kaeffer B, Uriel I G, Bottreau E
INRA, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France.
Cell Biol Int. 1994 Nov;18(11):1059-65. doi: 10.1006/cbir.1994.1029.
Temperature of culture can be used to modulate cellular metabolism for improving small intestinal cell culture and cryopreservation. An hypothermia pretreatment (2 days at 25 degrees C and 3 hours recovery at 37 degrees C) improved hamster cell survival to freeze-thaw damage (p < 0.01) but decreased the survival of 2 immortal pig ileal cell lines even though epithelioid IPI-2I cells were more tolerant to hypothermia than IPI-1 fibroblasts. Epithelioid cells survived 3 days at 25 degrees C with unaltered expression of cytokeratin-18 whereas colonies of fibroblasts did not survive more than a day at 25 degrees C (p < 0.001). These results suggest that hypothermia-tolerance of pig ileal cell lines might differ according to cell lineage calling for further experiments on small intestinal primary cell culture.
培养温度可用于调节细胞代谢，以改善小肠细胞培养和冷冻保存。低温预处理（25℃下处理2天，37℃下恢复3小时）可提高仓鼠细胞对冻融损伤的存活率（p < 0.01），但降低了2种永生猪回肠细胞系的存活率，尽管上皮样IPI-2I细胞比IPI-1成纤维细胞对低温更耐受。上皮样细胞在25℃下存活3天，细胞角蛋白-18的表达未改变，而成纤维细胞集落在25℃下存活不超过1天（p < 0.001）。这些结果表明，猪回肠细胞系的低温耐受性可能因细胞谱系而异，需要对小肠原代细胞培养进行进一步实验。
