Rice A, Boiron J M, Barbot C, Dupouy M, Dubosc-Marchenay N, Dumain P, Lacombe F, Reiffers J
Laboratoire de Greffe de Moelle, Université de Bordeaux II, France.
Exp Hematol. 1995 Apr;23(4):303-8.
We evaluated the expansion capacity of untreated and 5-fluorouracil (5-FU)-resistant peripheral blood stem cells (PBSC) after 7-day incubation with interleukin-1 (IL-1) plus IL-3 plus stem cell factor (SCF) or with medium alone. We found a significant increase in the proportion of CD34+ cells in the PBSC fraction resistant to 25 micrograms/mL 5-FU after 7-day incubation with IL-1 plus IL-3 plus SCF as compared with the untreated fraction (p = 0.011). We also showed that 5-FU-resistant PBSC have a greater capacity for expansion of IL-1/IL-3/SCF-responsive immature progenitors (p = 0.05), amplification of IL-3 plus GM-CSF responsive progenitors (p = 0.01), and production of committed single growth factor-responsive (granulocyte-macrophage colony-stimulating factor [GM-CSF]) precursors (p = 0.01) than the untreated PBSC. The expansion of all types of progenitors and CD34+ cells was only observed after 7-day incubation with IL-1 plus IL-3 plus SCF. These results suggest that PBSC contain a primitive stem cell population with an enhanced expansion capacity that is identified by 5-FU resistance. As these cells can be expanded in vitro, they may then be suitable for a number of clinical applications.
我们评估了未处理的和耐5-氟尿嘧啶（5-FU）的外周血干细胞（PBSC）在与白细胞介素-1（IL-1）加IL-3加干细胞因子（SCF）或仅与培养基一起孵育7天后的扩增能力。我们发现，与未处理的部分相比，耐5-FU的PBSC部分在与IL-1加IL-3加SCF孵育7天后，CD34+细胞的比例显著增加（p = 0.011）。我们还表明，耐5-FU的PBSC在扩增IL-1/IL-3/SCF反应性未成熟祖细胞方面能力更强（p = 0.05），在扩增IL-3加粒细胞-巨噬细胞集落刺激因子（GM-CSF）反应性祖细胞方面能力更强（p = 0.01），并且在产生定向的单一生长因子反应性（GM-CSF）前体细胞方面能力更强（p = 0.01），优于未处理的PBSC。仅在与IL-1加IL-3加SCF孵育7天后才观察到所有类型祖细胞和CD34+细胞的扩增。这些结果表明，PBSC含有一个原始干细胞群体，其扩增能力增强，可通过5-FU抗性来识别。由于这些细胞可以在体外扩增，它们可能适用于许多临床应用。
