用于直接检测粪便标本中大肠杆菌O157的免疫测定法的评估。
Evaluation of an immunoassay for direct detection of Escherichia coli O157 in stool specimens.
作者信息
Dylla B L, Vetter E A, Hughes J G, Cockerill F R
机构信息
Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.
出版信息
J Clin Microbiol. 1995 Jan;33(1):222-4. doi: 10.1128/jcm.33.1.222-224.1995.
Abstract
An enzyme-linked immunosorbent assay (ELISA) produced by LMD Laboratories, Inc., Carlsbad, Calif., was compared with culture for the detection of Escherichia coli O157. Nine of 185 stool specimens evaluated had positive results by the LMD E. coli O157 ELISA and grew E. coli O157 on culture; 174 had negative by LMD E. coli O157 ELISA results and did not grow E. coli O157 on culture. Of 174 specimens negative by LMD E. coli O157 ELISA, 117 specimens grew other enteric pathogens: Campylobacter spp. (46 isolates), Salmonella spp. (43 isolates), Yersinia spp. (20 isolates), and Shigella spp. (8 isolates). There were two indeterminant results by the LMD E. coli O157 ELISA. One stool specimen did not grow other enteric pathogens on culture, and one grew a Campylobacter sp. on culture. Both had negative LMD E. coli O157 ELISA results upon repeat testing. The LMD E. coli O157 ELISA is an accurate, easy-to-read screening method for the detection of E. coli O157 in fecal specimens.
摘要
对加利福尼亚州卡尔斯巴德市LMD实验室公司生产的一种酶联免疫吸附测定（ELISA）法与用于检测大肠杆菌O157的培养法进行了比较。在评估的185份粪便标本中，有9份经LMD大肠杆菌O157 ELISA检测结果呈阳性，且在培养中培养出大肠杆菌O157；174份经LMD大肠杆菌O157 ELISA检测结果为阴性，且在培养中未培养出大肠杆菌O157。在174份经LMD大肠杆菌O157 ELISA检测为阴性的标本中，有117份标本培养出其他肠道病原体：弯曲杆菌属（46株分离菌）、沙门氏菌属（43株分离菌）、耶尔森菌属（20株分离菌）和志贺氏菌属（8株分离菌）。LMD大肠杆菌O157 ELISA有两个不确定结果。一份粪便标本在培养中未培养出其他肠道病原体，另一份在培养中培养出一株弯曲杆菌。重复检测时，两者的LMD大肠杆菌O157 ELISA结果均为阴性。LMD大肠杆菌O157 ELISA是一种用于检测粪便标本中大肠杆菌O157的准确、易于判读的筛查方法。
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