多重聚合酶链反应在检测血性粪便中产生志贺毒素的非O157大肠杆菌中的应用：O26和O111血清群的鉴定
Application of multiplex PCR for detection of non-O157 verocytotoxin-producing Escherichia coli in bloody stools: identification of serogroups O26 and O111.
作者信息
Louie M, Read S, Simor A E, Holland J, Louie L, Ziebell K, Brunton J, Hii J
机构信息
Department of Microbiology, SD Laboratory Services, Sunnybrook Health Science Centre, Toronto, Ontario, Canada.
出版信息
J Clin Microbiol. 1998 Nov;36(11):3375-7. doi: 10.1128/JCM.36.11.3375-3377.1998.
Abstract
Primers were designed to amplify sequences of verocytotoxin genes and eaeA genes of Escherichia coli O26:H11, O111:H8, and O157:H7 in a multiplex PCR assay. This assay successfully detected E. coli O26:H11 in bloody stool specimens in which other enteric pathogens were not detected by culture-based methods. Rapid assays to detect non-O157:H7 verocytotoxin-producing E. coli is important to improve methods for the etiologic diagnosis of hemorrhagic colitis.
摘要
设计引物用于在多重聚合酶链反应（PCR）分析中扩增大肠杆菌O26:H11、O111:H8和O157:H7的志贺毒素基因和eaeA基因序列。该分析成功检测出血性粪便标本中的大肠杆菌O26:H11，而基于培养的方法未检测到其他肠道病原体。快速检测非O157:H7产志贺毒素大肠杆菌的分析对于改进出血性结肠炎的病因诊断方法很重要。
相似文献
1
Application of multiplex PCR for detection of non-O157 verocytotoxin-producing Escherichia coli in bloody stools: identification of serogroups O26 and O111.多重聚合酶链反应在检测血性粪便中产生志贺毒素的非O157大肠杆菌中的应用：O26和O111血清群的鉴定
J Clin Microbiol. 1998 Nov;36(11):3375-7. doi: 10.1128/JCM.36.11.3375-3377.1998.
2
Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains.在多重聚合酶链反应（PCR）检测中使用鞭毛H7基因作为靶点，并提高鉴定肠出血性大肠杆菌菌株的特异性。
J Clin Microbiol. 1997 Mar;35(3):656-62. doi: 10.1128/jcm.35.3.656-662.1997.
3
Detection and quantitation of enterohemorrhagic Escherichia coli O157, O111, and O26 in beef and bovine feces by real-time polymerase chain reaction.通过实时聚合酶链反应检测和定量牛肉及牛粪中的肠出血性大肠杆菌O157、O111和O26 。
J Food Prot. 2002 Sep;65(9):1371-80. doi: 10.4315/0362-028x-65.9.1371.
4
Semi-automated fluorogenic PCR assays (TaqMan) forrapid detection of Escherichia coli O157:H7 and other shiga toxigenic E. coli.用于快速检测大肠杆菌O157:H7和其他产志贺毒素大肠杆菌的半自动荧光定量PCR检测法（TaqMan法）
Mol Cell Probes. 1999 Aug;13(4):291-302. doi: 10.1006/mcpr.1999.0251.
5
Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers.eae基因的序列异质性以及使用血清型特异性引物检测产志贺毒素大肠杆菌
Epidemiol Infect. 1994 Jun;112(3):449-61. doi: 10.1017/s0950268800051153.
6
Genetic analysis for virulence factors in Escherichia coli O104:H21 that was implicated in an outbreak of hemorrhagic colitis.对与出血性结肠炎暴发有关的大肠杆菌O104:H21毒力因子的基因分析。
J Clin Microbiol. 2001 Jan;39(1):24-8. doi: 10.1128/JCM.39.1.24-28.2001.
7
Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157.通过使用针对stx1、stx2、eaeA、肠出血性大肠杆菌hlyA、rfbO111和rfbO157的多重聚合酶链反应（PCR）检测法来检测和鉴定志贺毒素产生型大肠杆菌并进行特性分析。
J Clin Microbiol. 1998 Feb;36(2):598-602. doi: 10.1128/JCM.36.2.598-602.1998.
8
Detection of Escherichia coli O157:H7 by multiplex PCR.通过多重聚合酶链反应检测大肠杆菌O157:H7
J Clin Microbiol. 1995 Aug;33(8):2188-91. doi: 10.1128/jcm.33.8.2188-2191.1995.
9
A multiplex PCR for identifying Shiga-like toxin-producing Escherichia coli O157:H7.一种用于鉴定产志贺样毒素大肠杆菌O157:H7的多重聚合酶链反应。
Lett Appl Microbiol. 1997 Mar;24(3):172-6. doi: 10.1046/j.1472-765x.1997.00375.x.
10
The detection of Shiga toxin-producing Escherichia coli in diagnostic bovine faecal samples using vancomycin-cefixime-cefsulodin blood agar and PCR.使用万古霉素-头孢克肟-头孢磺啶血琼脂和聚合酶链反应检测诊断用牛粪便样本中产志贺毒素大肠杆菌
FEMS Microbiol Lett. 2001 Apr 20;198(1):17-22. doi: 10.1111/j.1574-6968.2001.tb10613.x.
引用本文的文献
1
Development of dual reporter imaging system for to monitor the spatio-temporal pathogenesis and vaccine efficacy.用于监测时空发病机制和疫苗效力的双报告基因成像系统的开发。
Clin Exp Vaccine Res. 2018 Jul;7(2):129-138. doi: 10.7774/cevr.2018.7.2.129. Epub 2018 Jul 31.
2
Topological data analysis of Escherichia coli O157:H7 and non-O157 survival in soils.大肠杆菌O157:H7与非O157在土壤中存活情况的拓扑数据分析
Front Cell Infect Microbiol. 2014 Sep 5;4:122. doi: 10.3389/fcimb.2014.00122. eCollection 2014.
3
Luminescence based enzyme-labeled phage (Phazyme) assays for rapid detection of Shiga toxin producing Escherichia coli serogroups.基于发光的酶标记噬菌体（Phazyme）检测法用于快速检测产志贺毒素大肠杆菌血清群。
Bacteriophage. 2011 Mar;1(2):101-110. doi: 10.4161/bact.1.2.15666.
4
Epidemiological survey on Escherichia coli O157 in Chongqing and Three-Gorge Reservoir Areas of China.中国重庆及三峡库区大肠埃希菌O157流行病学调查
Vet Res Commun. 2008 Aug;32(6):449-61. doi: 10.1007/s11259-008-9048-8. Epub 2008 May 15.
5
6
Molecular serotyping of Escherichia coli O26:H11.大肠杆菌O26:H11的分子血清分型
Appl Environ Microbiol. 2005 Aug;71(8):4941-4. doi: 10.1128/AEM.71.8.4941-4944.2005.
7
Shiga toxin-producing Escherichia coli in the feces of Alberta feedlot cattle.艾伯塔省饲养场牛粪便中产志贺毒素大肠杆菌
Can J Vet Res. 2004 Apr;68(2):150-3.
8
PCR detection of Escherichia coli O157:H7 directly from stools: evaluation of commercial extraction methods for purifying fecal DNA.直接从粪便中进行大肠杆菌O157:H7的PCR检测：用于纯化粪便DNA的商业提取方法评估
J Clin Microbiol. 2000 Nov;38(11):4108-13. doi: 10.1128/JCM.38.11.4108-4113.2000.
9
The role of DNA amplification technology in the diagnosis of infectious diseases.DNA扩增技术在传染病诊断中的作用。
CMAJ. 2000 Aug 8;163(3):301-9. doi: 10.1016/s1381-1169(00)00220-x.
本文引用的文献
1
Sensitivities and specificities of premier E. coli O157 and premier EHEC enzyme immunoassays for diagnosis of infection with verotxin (Shiga-like toxin)-producing Escherichia coli. The SYNSORB Pk Study investigators.用于诊断产志贺毒素（类志贺毒素）大肠杆菌感染的首要大肠杆菌O157和首要肠出血性大肠杆菌酶免疫测定的敏感性和特异性。SYNSORB Pk研究调查人员。
J Clin Microbiol. 1998 Jun;36(6):1608-11. doi: 10.1128/JCM.36.6.1608-1611.1998.
2
Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157.通过使用针对stx1、stx2、eaeA、肠出血性大肠杆菌hlyA、rfbO111和rfbO157的多重聚合酶链反应（PCR）检测法来检测和鉴定志贺毒素产生型大肠杆菌并进行特性分析。
J Clin Microbiol. 1998 Feb;36(2):598-602. doi: 10.1128/JCM.36.2.598-602.1998.
3
Shiga toxin-producing Escherichia coli serotype OX3:H21 as a cause of hemolytic-uremic syndrome.产志贺毒素大肠杆菌血清型OX3:H21作为溶血尿毒综合征的一个病因
Clin Infect Dis. 1997 Jun;24(6):1278-9; discussion 1280-2. doi: 10.1086/513668.
4
Perspective: the problem of non-O157:H7 shiga toxin (Verocytotoxin)-producing Escherichia coli.观点：非O157:H7产志贺毒素（Vero细胞毒素）大肠杆菌问题
J Infect Dis. 1996 Nov;174(5):1136-9. doi: 10.1093/infdis/174.5.1136.
5
Immune response to non-O157 Vero toxin-producing Escherichia coli in patients with hemolytic uremic syndrome.溶血尿毒综合征患者对非O157产志贺毒素大肠杆菌的免疫反应。
J Infect Dis. 1996 Nov;174(5):1028-39. doi: 10.1093/infdis/174.5.1028.
6
Enterohemolytic phenotypes and genotypes of shiga toxin-producing Escherichia coli O111 strains from patients with diarrhea and hemolytic-uremic syndrome.腹泻和溶血尿毒综合征患者中产志贺毒素大肠杆菌O111菌株的肠溶血表型和基因型
J Clin Microbiol. 1996 Oct;34(10):2364-7. doi: 10.1128/jcm.34.10.2364-2367.1996.
7
Neutralizing antibodies to Escherichia coli Vero cytotoxin 1 and antibodies to O157 lipopolysaccharide in healthy farm family members and urban residents.健康农场家庭成员和城市居民体内针对大肠杆菌Vero细胞毒素1的中和抗体及针对O157脂多糖的抗体。
J Clin Microbiol. 1996 Sep;34(9):2053-7. doi: 10.1128/jcm.34.9.2053-2057.1996.
8
Detection and characterization of the eae gene of Shiga-like toxin-producing Escherichia coli using polymerase chain reaction.利用聚合酶链反应检测和鉴定产志贺样毒素大肠杆菌的eae基因
J Clin Microbiol. 1993 May;31(5):1268-74. doi: 10.1128/jcm.31.5.1268-1274.1993.
9
Direct detection of Escherichia coli Shiga-like toxin genes in primary fecal cultures by polymerase chain reaction.通过聚合酶链反应直接检测原代粪便培养物中的大肠杆菌志贺样毒素基因。
J Clin Microbiol. 1993 Nov;31(11):3063-7. doi: 10.1128/jcm.31.11.3063-3067.1993.
10
Sequence heterogeneity of the eae gene and detection of verotoxin-producing Escherichia coli using serotype-specific primers.eae基因的序列异质性以及使用血清型特异性引物检测产志贺毒素大肠杆菌
Epidemiol Infect. 1994 Jun;112(3):449-61. doi: 10.1017/s0950268800051153.
Zotero 插件