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多重聚合酶链反应在检测血性粪便中产生志贺毒素的非O157大肠杆菌中的应用：O26和O111血清群的鉴定

Application of multiplex PCR for detection of non-O157 verocytotoxin-producing Escherichia coli in bloody stools: identification of serogroups O26 and O111.

作者信息

Louie M, Read S, Simor A E, Holland J, Louie L, Ziebell K, Brunton J, Hii J

机构信息

Department of Microbiology, SD Laboratory Services, Sunnybrook Health Science Centre, Toronto, Ontario, Canada.

出版信息

J Clin Microbiol. 1998 Nov;36(11):3375-7. doi: 10.1128/JCM.36.11.3375-3377.1998.

DOI:10.1128/JCM.36.11.3375-3377.1998
PMID:9774599
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC105335/
Abstract

Primers were designed to amplify sequences of verocytotoxin genes and eaeA genes of Escherichia coli O26:H11, O111:H8, and O157:H7 in a multiplex PCR assay. This assay successfully detected E. coli O26:H11 in bloody stool specimens in which other enteric pathogens were not detected by culture-based methods. Rapid assays to detect non-O157:H7 verocytotoxin-producing E. coli is important to improve methods for the etiologic diagnosis of hemorrhagic colitis.

摘要

设计引物用于在多重聚合酶链反应（PCR）分析中扩增大肠杆菌O26:H11、O111:H8和O157:H7的志贺毒素基因和eaeA基因序列。该分析成功检测出血性粪便标本中的大肠杆菌O26:H11，而基于培养的方法未检测到其他肠道病原体。快速检测非O157:H7产志贺毒素大肠杆菌的分析对于改进出血性结肠炎的病因诊断方法很重要。

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