Sarwar M, Tomiyoshi K, Inoue T, Fukazawa K, Endo K
Department of Nuclear Medicine, Gunma University School of Medicine, Japan.
Ann Nucl Med. 1994 Nov;8(4):301-6. doi: 10.1007/BF03165035.
Serum levels of cytokeratin subunit 19 (CYFRA 21-1) were measured in 42 healthy volunteers, 104 cases of malignant diseases, 30 patients with chronic renal failure and 13 patients with nonmalignant and infectious diseases. The reliability of the method was demonstrated after dilution of serum samples and intra- and inter-assay reproducibility. Serum CYFRA-21-1 concentrations were less than 2.00 ng/ml in all healthy controls and 86% of the malignant cases had high serum CYFRA 21-1 levels. However slightly elevated values of CYFRA 21-1 were observed in most chronic renal failure patients. High correlation was observed between serum CYFRA 21-1 and Tissue Polypeptide Antigen (TPA) values (r = 0.90, n = 10) but not with serum alpha-feto protein (AFP) concentrations. Furthermore, cross binding tests with the CYFRA 21-1 tracer/CYFRA 21-1 antibody-coated beads and CYFRA 21-1 tracer/TPA antibody-coated beads also gave an almost linear graph. These results indicate that CYFRA 21-1 and TPA share similar type of antigens.
对42名健康志愿者、104例恶性疾病患者、30例慢性肾衰竭患者及13例非恶性感染性疾病患者测定了细胞角蛋白19亚基（CYFRA 21-1）的血清水平。在对血清样本进行稀释以及进行批内和批间重复性试验后，证明了该方法的可靠性。所有健康对照者的血清CYFRA-21-1浓度均低于2.00 ng/ml，86%的恶性疾病患者血清CYFRA 21-1水平较高。然而，大多数慢性肾衰竭患者的CYFRA 21-1值略有升高。血清CYFRA 21-1与组织多肽抗原（TPA）值之间存在高度相关性（r = 0.90，n = 10），但与血清甲胎蛋白（AFP）浓度无关。此外，用CYFRA 21-1示踪剂/CYFRA 21-1抗体包被磁珠和CYFRA 21-1示踪剂/TPA抗体包被磁珠进行的交叉结合试验也得到了几乎呈线性的图表。这些结果表明CYFRA 21-1和TPA具有相似类型的抗原。
