肿瘤标志物TPA、TPS、TPACYK和CYFRA 21-1与角蛋白8、18和19的反应不同。

The tumor markers TPA, TPS, TPACYK and CYFRA 21-1 react differently with the keratins 8, 18 and 19.

作者信息

Bodenmüller H, Donié F, Kaufmann M, Banauch D

机构信息

Boehringer Mannheim GmbH, Research Center Tutzing, Germany.

出版信息

Int J Biol Markers. 1994 Apr-Jun;9(2):70-4. doi: 10.1177/172460089400900202.

DOI:10.1177/172460089400900202

PMID:7523544

Abstract

The commercially available tumor marker tests TPA, TPS, TPACYK and CYFRA 21-1 react with simple epithelium keratins. From clinical studies it can be deduced that the pattern of keratin recognition must be different for each of these tests. We therefore studied the reactivity of the keratin fragment combinations K8/K18 and K8/K19 in the different tests and determined the reactivity of the corresponding soluble antibodies with purified keratin 8, 18 and 19 in immunoblots. TPS and CYFRA 21-1 were found to distinguish clearly between the keratin fragment combinations K8/K18 (TPS) and K8/K19 (CYFRA 21-1). TPA and TPACYK reacted with both combinations, however, with different intensities. On immunoblots the CYFRA 21-1 antibodies reacted exclusively with K19, whereas the antibodies of the other assays reacted with at least 2 of the keratins investigated.

摘要

市售的肿瘤标志物检测项目TPA、TPS、TPACYK和CYFRA 21-1与单层上皮角蛋白发生反应。从临床研究可以推断，这些检测项目中每种对角蛋白的识别模式必然不同。因此，我们研究了角蛋白片段组合K8/K18和K8/K19在不同检测项目中的反应性，并在免疫印迹中测定了相应可溶性抗体与纯化的角蛋白8、18和19的反应性。结果发现，TPS和CYFRA 21-1能够清楚地区分角蛋白片段组合K8/K18（TPS）和K8/K19（CYFRA 21-1）。然而，TPA和TPACYK与这两种组合都发生反应，但强度不同。在免疫印迹中，CYFRA 21-1抗体仅与K19发生反应，而其他检测方法的抗体与至少两种所研究的角蛋白发生反应。