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Nonradioactive 3'-end-labeling of RNA molecules of different lengths by terminal deoxynucleotidyltransferase.

作者信息

Rosemeyer V, Laubrock A, Seibl R

机构信息

Boehringer Mannheim GmbH, Penzberg, Federal Republic of Germany.

出版信息

Anal Biochem. 1995 Jan 1;224(1):446-9. doi: 10.1006/abio.1995.1068.

DOI:10.1006/abio.1995.1068
PMID:7535988
Abstract

Terminal deoxynucleotidyltransferase is well known for its ability to add nucleotides to the 3'-ends of DNA; here we demonstrate a corresponding activity with RNA as acceptor molecule. Using DIG- or biotin-dUTP as substrate, a tailing reaction is used for nonradioactive 3'-end-labeling of RNA oligonucleotides, isolated RNA molecules such as tRNA from E. coli, and MS2 RNA as well as in vitro transcripts. The tail length and associated sensitivity of detection can be varied using DIG-ddUTP, DIG-dUTP, or a nucleotide mixture of DIG-dUTP and dATP.

摘要

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