利用酵母多聚腺苷酸聚合酶和各种核苷酸对RNA进行加尾和3'末端标记。
Tailing and 3'-end labeling of RNA with yeast poly(A) polymerase and various nucleotides.
作者信息
Martin G, Keller W
机构信息
Department of Cell Biology, Biozentrum, University of Basel, Switzerland.
出版信息
RNA. 1998 Feb;4(2):226-30.
Abstract
We have tested conditions for the labeling and tailing the 3'-end of RNAs with yeast poly(A) polymerase. Conditions were optimized for addition of NTP, dNTP, or ddNTP nucleotides to RNA. ATP, GTP, and UTP were useful for adding homopolymer tracts of various lengths. The nonradioactive nucleotides biotin-N6-ATP and digoxigenin-11-UTP also were used efficiently.
摘要
我们已经测试了用酵母多聚腺苷酸聚合酶对RNA的3'末端进行标记和加尾的条件。对向RNA中添加NTP、dNTP或ddNTP核苷酸的条件进行了优化。ATP、GTP和UTP可用于添加各种长度的同聚物片段。非放射性核苷酸生物素-N6-ATP和地高辛-11-UTP也能有效使用。
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