Stim K P
Department of Biochemistry and Cell Biology, Rice University, Houston, TX 77005-1892.
Mol Ecol. 1995 Feb;4(1):1-10. doi: 10.1111/j.1365-294x.1995.tb00186.x.
Three methods were used to provide information on the identity and phylogenetic relatedness of 19 aerobic, chemoheterotrophic bacteria isolated from topsoil and deep subsurface sediments at a site in South Carolina. These methods were (i) analysis of selected physiological traits, (ii) restriction endonuclease analysis (REA) of genomic DNA, and (iii) analysis of 16S ribosomal RNA sequences. When the 16S rRNA sequences were compared with those for 12 standard strains, two topsoil isolates and six subsurface strains formed a tight group with the high-G+C Gram-positive bacteria and appeared to be most closely related to Arthrobacter globiformis--a coryneform-actinomycete bacterium with unusually effective survival capabilities. The rest of the subsurface isolates were scattered among the standard strains from the Proteobacteria-including the pseudomonads and Agrobacterium tumefaciens--or the low-G+C Gram-positive bacteria.
采用了三种方法来提供关于从南卡罗来纳州某一地点的表土和深层地下沉积物中分离出的19株需氧、化能异养细菌的身份和系统发育相关性的信息。这些方法包括：（i）对选定生理特征的分析；（ii）基因组DNA的限制性内切酶分析（REA）；（iii）16S核糖体RNA序列分析。当将16S rRNA序列与12株标准菌株的序列进行比较时，两株表土分离株和六株地下菌株与高G+C革兰氏阳性菌形成了一个紧密的群体，并且似乎与球形节杆菌（一种具有异常有效生存能力的棒状放线菌）关系最为密切。其余的地下分离株则分散在变形菌门的标准菌株中，包括假单胞菌和根癌农杆菌，或者低G+C革兰氏阳性菌中。
