Fredrickson J K, Balkwill D L, Drake G R, Romine M F, Ringelberg D B, White D C
Pacific Northwest Laboratory, Richland, Washington 99352, USA.
Appl Environ Microbiol. 1995 May;61(5):1917-22. doi: 10.1128/aem.61.5.1917-1922.1995.
An obligately aerobic chemoheterotrophic bacterium (strain F199) previously isolated from Southeast Coastal Plain subsurface sediments and shown to degrade toluene, naphthalene, and other aromatic compounds (J. K. Fredrickson, F. J. Brockman, D. J. Workman, S. W. Li, and T. O. Stevens, Appl. Environ. Microbiol. 57:796-803, 1991) was characterized by analysis of its 16S rRNA nucleotide base sequence and cellular lipid composition. Strain F199 contained 2-OH14:0 and 18:1 omega 7c as the predominant cellular fatty acids and sphingolipids that are characteristic of the genus Sphingomonas. Phylogenetic analysis of its 16S rRNA sequence indicated that F199 was most closely related to Sphingomonas capsulata among the bacteria currently in the Ribosomal Database. Five additional isolates from deep Southeast Coastal Plain sediments were determined by 16S rRNA sequence analysis to be closely related to F199. These strains also contained characteristic sphingolipids. Four of these five strains could also grow on a broad range of aromatic compounds and could mineralize [14C]toluene and [14C]naphthalene. S. capsulata (ATCC 14666), Sphingomonas paucimobilis (ATCC 29837), and one of the subsurface isolates were unable to grow on any of the aromatic compounds or mineralize toluene or naphthalene. These results indicate that bacteria within the genus Sphingomonas are present in Southeast Coastal Plain subsurface sediments and that the capacity for degrading a broad range of substituted aromatic compounds appears to be common among Sphingomonas species from this environment.
一种专性需氧化学异养细菌（菌株F199），先前从东南沿海平原地下沉积物中分离得到，并已证明其能够降解甲苯、萘和其他芳香族化合物（J.K.弗雷德里克森、F.J.布罗克曼、D.J.沃克曼、S.W.李和T.O.史蒂文斯，《应用与环境微生物学》57:796 - 803，1991年），通过对其16S rRNA核苷酸碱基序列和细胞脂质组成的分析对其进行了表征。菌株F199含有2 - OH14:0和18:1 ω7c作为主要的细胞脂肪酸以及鞘脂，这些是鞘氨醇单胞菌属的特征。对其16S rRNA序列的系统发育分析表明，在核糖体数据库目前收录的细菌中，F199与荚膜鞘氨醇单胞菌关系最为密切。通过16S rRNA序列分析确定，另外五株从东南沿海平原深层沉积物中分离得到的菌株与F199密切相关。这些菌株也含有特征性鞘脂。这五株菌株中的四株还能够在多种芳香族化合物上生长，并能使[14C]甲苯和[14C]萘矿化。荚膜鞘氨醇单胞菌（ATCC 14666）、少动鞘氨醇单胞菌（ATCC 29837）以及一株地下分离菌株不能在任何一种芳香族化合物上生长，也不能使甲苯或萘矿化。这些结果表明，鞘氨醇单胞菌属的细菌存在于东南沿海平原地下沉积物中，并且在来自该环境的鞘氨醇单胞菌物种中，降解多种取代芳香族化合物的能力似乎很常见。
