Too H P, Marriott D R, Wilkin G P
Department of Biochemistry, National University of Singapore, Kent Ridge Crescent.
Neurosci Lett. 1994 Dec 5;182(2):185-7. doi: 10.1016/0304-3940(94)90793-5.
The presence of mRNA transcripts coding for preprotachykinin-A and the substance P receptor in cultured astrocytes is demonstrated by a combination of reverse transcription/polymerase chain reaction (PCR) and Southern blotting. These findings showed that astrocytes in culture are capable of synthesing both the precursor of substance P (preprotachykinin-A) and the cognate receptor, substance P receptor (NK1). The simultaneous presence of both the ligand (substance P) and the receptor (NK1) may indicate an autocrine nature of astrocyte communication.
通过逆转录/聚合酶链反应（PCR）和Southern印迹法相结合，证实了培养的星形胶质细胞中存在编码前速激肽原A和P物质受体的mRNA转录本。这些发现表明，培养的星形胶质细胞能够合成P物质的前体（前速激肽原A）和同源受体P物质受体（NK1）。配体（P物质）和受体（NK1）同时存在可能表明星形胶质细胞通讯具有自分泌性质。
