Huang Y T, Lo J W, Lin H C, Tsai Y T, Hong C Y, Yang M C
Institute of Traditional Medicine, National Yang Ming Medical College, Taipei, ROC.
Pharmacology. 1995 Feb;50(2):86-91. doi: 10.1159/000139270.
The purpose of this study was to investigate the possible changes of cyclic nucleotide contents in portal hypertensive rats. Portal hypertension was induced by partial portal vein ligation (PVL) in Sprague-Dawley rats. Sham-operated rats served as controls. Hemodynamic and cyclic nucleotide measurements were performed at 14 days after surgery. The portal venous pressure was significantly higher, while systemic arterial pressure and heart rate were lower in PVL rats than those in controls. Basal cAMP (PVL, 10.91 +/- 0.98, vs. sham, 8.08 +/- 0.81 pmol/mg protein) and cGMP (PVL, 0.91 +/- 0.12, vs. sham, 0.59 +/- 0.05 pmol/mg protein) contents in the tail artery were significantly higher in PVL rats. Isobutyryl methylxanthine (10(-5) M), a nonspecific phosphodiesterase inhibitor, exerted similarly stimulating effects on the tissue cGMP (PVL, 158 +/- 10, vs. sham, 178 +/- 20%) and cGMP (295 +/- 28 vs. 316 +/- 71%) levels in both PVL and control rats; so did forskolin (10(-6) M) on the cAMP (184 +/- 20 vs. 197 +/- 66%) content in both groups. Our results showed that the arterial cAMP and cGMP contents were higher in PVL rats, which may contribute to the reduction of peripheral resistance in portal hypertension.
本研究的目的是探讨门静脉高压大鼠体内环核苷酸含量可能发生的变化。通过对Sprague-Dawley大鼠进行部分门静脉结扎（PVL）诱导门静脉高压。假手术大鼠作为对照。在术后14天进行血流动力学和环核苷酸测量。PVL大鼠的门静脉压力显著升高，而全身动脉压和心率低于对照组。PVL大鼠尾动脉中的基础cAMP（PVL组为10.91±0.98，假手术组为8.08±0.81 pmol/mg蛋白）和cGMP（PVL组为0.91±0.12，假手术组为0.59±0.05 pmol/mg蛋白）含量显著更高。非特异性磷酸二酯酶抑制剂异丁基甲基黄嘌呤（10⁻⁵ M）对PVL大鼠和对照大鼠的组织cGMP水平（PVL组为158±10，假手术组为178±20%）和cGMP水平（295±28对316±71%）具有类似的刺激作用；10⁻⁶ M的福斯高林对两组的cAMP含量（184±20对197±66%）也有类似作用。我们的结果表明，PVL大鼠动脉中的cAMP和cGMP含量较高，这可能有助于降低门静脉高压时的外周阻力。
