Onoviran O, Adegboye D S, Makinde A A, Chima J C
Dev Biol Stand. 1978;41:361-5.
A lyophilised T1 vaccine was produced by direct lyophilisation of a 72-hour broth culture to which 10% sterile sucrose solution was added. Each vial contained 2 ml to make a total of 10 doses when reconstituted. Each ml of the product contained an average of 109 colony forming units of the organism. When injected at the tip of the tail or subcutaneously behind the shoulder of 2,000 zebu and 1,0000 Ndama cattle, no outward reactions were observed. 12 zebu cattle were vaccinated and challenged 6 months later by direct contact with artificially infected cattle for 4 months. All the animals were completely protected against the disease.
