Costantini P, Chernyak B V, Petronilli V, Bernardi P
CNR Unit for the Study of Physiology of Mitochondria, University of Padova Medical School, Italy.
FEBS Lett. 1995 Apr 3;362(2):239-42. doi: 10.1016/0014-5793(95)00256-9.
In this paper we introduce monobromobimane, a thiol reagent, as a selective blocker of the recently identified dithiol whose oxidation-reduction status modifies voltage sensing by the mitochondrial permeability transition pore, a cyclosporin A-sensitive channel. Monobromobimane does not inhibit the phosphate carrier, nor does it interfere with Ca2+ transport, energy coupling or ATP production and transport. We show that monobromobimane selectively prevents the shift in pore gating potential caused by some dithiol oxidants or crosslinkers but not by increasing [Ca2+], allowing a clear distinction of the pore agonists which act at this site.
在本文中，我们介绍了一种硫醇试剂——一溴代双硫丹，它是最近发现的二硫醇的选择性阻滞剂，该二硫醇的氧化还原状态通过线粒体通透性转换孔（一种对环孢菌素A敏感的通道）来调节电压传感。一溴代双硫丹不抑制磷酸载体，也不干扰Ca2+转运、能量偶联或ATP的产生和转运。我们表明，一溴代双硫丹能选择性地阻止某些二硫醇氧化剂或交联剂引起的孔门控电位的变化，但不能阻止因[Ca2+]增加而引起的变化，从而能够清晰地区分作用于该位点的孔激动剂。
