Ren Y, Silverstein R L, Allen J, Savill J
Department of Medicine, University Hospital, Nottingham, United Kingdom.
J Exp Med. 1995 May 1;181(5):1857-62. doi: 10.1084/jem.181.5.1857.
Phagocyte recognition and ingestion of intact cells undergoing apoptosis are key events in this generally important program of cell death. Insufficient phagocyte capacity for apoptotic cells can result in failure to clear dying cells before membrane integrity is lost, resulting in leakage of noxious cell contents and severe tissue damage. However, no means has been available to increase phagocytic clearance of apoptotic cells. We now report that transfection of the macrophage adhesion molecule CD36 into human Bowes melanoma cells specifically conferred greatly increased capacity to ingest apoptotic neutrophils, lymphocytes, and fibroblasts, comparable to that exhibited by macrophages. Furthermore, when CD36 was transfected into another cell type with limited capacity to take up apoptotic bodies, the monkey COS-7 cell, similar effects were observed. Therefore, CD36 gene transfer can confer "professional" capacity to ingest apoptotic cells upon "amateur" phagocytes.
吞噬细胞识别并摄取正在经历凋亡的完整细胞是这一普遍重要的细胞死亡程序中的关键事件。吞噬细胞对凋亡细胞的处理能力不足会导致在细胞膜完整性丧失之前无法清除垂死细胞，从而导致有害细胞内容物泄漏和严重的组织损伤。然而，一直没有办法增加对凋亡细胞的吞噬清除。我们现在报告，将巨噬细胞粘附分子CD36转染到人鲍斯黑色素瘤细胞中，可特异性地使其摄取凋亡中性粒细胞、淋巴细胞和成纤维细胞的能力大大增强，与巨噬细胞表现出的能力相当。此外，当将CD36转染到另一种摄取凋亡小体能力有限的细胞类型——猴COS-7细胞中时，也观察到了类似的效果。因此，CD36基因转移可赋予“业余”吞噬细胞“专业”摄取凋亡细胞的能力。
