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鸟类和哺乳动物神经胶质细胞中的凝集素结合位点。

Lectin binding sites in glia of birds and mammals.

作者信息

Garcia-Segura L M, Martinez-Rodriguez R, Martinez-Murillo R, Suarez I

出版信息

Gegenbaurs Morphol Jahrb. 1978;124(5):637-48.

PMID:753685
Abstract

Concanavalin A binding sites are studied in sections of nervous tissue from Central Nervous System of birds and mammals, paying special attention to the glial cells. The Con A-PO-DAB sequence for glycoproteins demonstration described by Bernhard and Avrameas (1971) was used. The highest concentration of Con A binding sites is demonstrated in the cells of the interfascicular oligodendroglia. Positivity resulting with the use of the Con A-PO-DAB serquence is much intenser in the oligodendroglia cells than in neurons. Ependymal cells also showed positivity specially at level of the perinuclear region. The intercellular space also presented Con A binding sites. All the cells of the interfascicular oligodendroglia presented the same characteristics, both in diverse localizations of the Nervous System of a certain species and in the different species studied.

摘要

对鸟类和哺乳动物中枢神经系统神经组织切片中的伴刀豆球蛋白A结合位点进行了研究,特别关注神经胶质细胞。采用了Bernhard和Avrameas(1971年)描述的用于糖蛋白显示的伴刀豆球蛋白A-过氧化物酶-二氨基联苯胺序列。在束间少突胶质细胞中显示出最高浓度的伴刀豆球蛋白A结合位点。使用伴刀豆球蛋白A-过氧化物酶-二氨基联苯胺序列产生的阳性反应在少突胶质细胞中比在神经元中强烈得多。室管膜细胞也显示出阳性,特别是在核周区域水平。细胞间隙也存在伴刀豆球蛋白A结合位点。束间少突胶质细胞的所有细胞都呈现相同的特征,无论是在某一物种神经系统的不同定位中,还是在所研究的不同物种中。

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