Garcia-Segura L M, Martinez-Rodriguez R, Bogonez E, Martinez-Murillo R, Toledano A
J Hirnforsch. 1978;19(5):423-32.
Cerebellum glycoproteins of rats and ducks are studied using sugar-specific agglutinins. It is used the Con A-Peroxidase-DAB technique described by Bernhard and Avrameas (1971). Other agglutinins (Tetragonolobus Purpureus Lectin, Wheat Germ Agglutinin and Soybean Agglutinin) fixed to Peroxidase according to the technique of Gonatas and Avrameas (1973) are also used. The techniques for mucopolysaccharides as described Martínez-Rodriguez et al., (1976) are carried out as well. By means of all these techniques, results that suggest the existence of metabolic cycles in Purkinje cells are obtained.
使用糖特异性凝集素对大鼠和鸭的小脑糖蛋白进行了研究。采用了Bernhard和Avrameas(1971年)描述的Con A-过氧化物酶-DAB技术。还使用了根据Gonatas和Avrameas(1973年)的技术固定在过氧化物酶上的其他凝集素(紫花四棱豆凝集素、麦胚凝集素和大豆凝集素)。Martínez-Rodríguez等人(1976年)描述的用于粘多糖的技术也得以实施。通过所有这些技术,获得了提示浦肯野细胞中存在代谢循环的结果。