Kruglova E B, Krasnitskaia A A, Maleev V Ia
Mol Biol (Mosk). 1995 Jan-Feb;29(1):125-34.
The spectrophotometric titration curves of the complexes of polynucleotide matrix with pyronine G are highly specific depending on the type and conformation of polyelectrolyte (native and denatured DNA, polyribonucleotides, tRNA). It is found that DNA from the liver of experimental animals of different age, permanent inhabitants of the Chernobyl zone, forms complexes with RNA. A method for testing the defects in a DNA secondary structure have been developed based on comparative analysis of the spectra for the complexes of different nucleic acids with dyes and relative curves of spectrophotometric titration.
多核苷酸基质与派洛宁G形成的配合物的分光光度滴定曲线高度特异，取决于聚电解质的类型和构象（天然和变性DNA、聚核糖核苷酸、tRNA）。研究发现，来自不同年龄实验动物肝脏的DNA、切尔诺贝利地区的常住居民的DNA会与RNA形成配合物。基于对不同核酸与染料形成的配合物的光谱以及分光光度滴定的相关曲线进行比较分析，开发出了一种检测DNA二级结构缺陷的方法。
