Blagoĭ Iu P, Kornilova S V, Leont'ev V S, Sorokin V A, Gladchenko G O, Valeev V A, Grigor'ev D N, Kapinos L E, Bondarenko V N, Kolod V Ia
Biofizika. 1994 Jul-Aug;39(4):637-45.
The properties of animal DNAs exposed to prolonged irradiation in the Chernobyl zone, have been studied by the methods of viscometry, thermal denaturation, IR-spectroscopy, and electrophoresis. High content of low-molecular fractions have been observed in the preparations of DNA from liver and spleen, their quantities increasing with age and generation of animals. This effect is especially strong in DNA from liver. Low-molecular fraction of DNA is shown to be enriched with G-C pairs and to consist of the following four fractions: 1) approximately 500 base pairs (B.p.), 2) approximately 1.5 divided by 2 thousand B.p., 3) approximately 4 divided by 5 thousand B.p. and 4) a mixture of approximately 20 thousand B.p. fragments. Further, it has been observed that the DNA preparations from the tissues of experimental animals contain about ten times higher contents of Fe, Zn, Se and other elements as compared to the control preparations.
利用粘度测定法、热变性法、红外光谱法和电泳法，对切尔诺贝利地区经长时间辐射的动物DNA的特性进行了研究。在肝脏和脾脏的DNA制剂中观察到低分子部分的含量很高，其数量随动物年龄和代数的增加而增加。这种效应在肝脏DNA中尤为明显。结果表明，DNA的低分子部分富含G-C碱基对，由以下四个部分组成：1）约500个碱基对（B.p.），2）约1.5除以2千个B.p.，3）约4除以5千个B.p.，4）约2万个B.p.片段的混合物。此外，还观察到，与对照制剂相比，实验动物组织的DNA制剂中Fe、Zn、Se和其他元素的含量高出约10倍。
