Barrera J M, Francis B, Ercilla G, Nelles M, Achord D, Darner J, Lee S R
Hospital Clinic I Provincial de Barcelona, Spain.
Vox Sang. 1995;68(1):15-8. doi: 10.1111/j.1423-0410.1995.tb02538.x.
The sensitivity of ORTHO HCV 3.0 ELISA Test System (ELISA 3) for the detection of anti-HCV was compared with the second-generation ELISA, OR-THO HCV 2.0 ELISA Test System (ELISA 2). ELISA 3 differs from ELISA 2 in that it incorporates the HCV recombinant antigen NS5, in addition to recombinant antigens derived from the NS3, NS4 and core regions of the HCV genome. Specimens tested consisted of serial bleeds obtained from 21 individuals undergoing seroconversion following acquisition of post-transfusion HCV infection. ELISA 3 demonstrated significantly greater sensitivity than ELISA 2, detecting seroconversion earlier in 24% (5/21) of cases. Although one of these cases appeared to represent early seroconversion to NS5, most of the improved sensitivity of ELISA 3 appeared to derive from increased detectability of anti-c33c.
将ORTHO HCV 3.0酶联免疫吸附测定试验系统（ELISA 3）检测抗丙型肝炎病毒（anti-HCV）的灵敏度与第二代酶联免疫吸附测定法即ORTHO HCV 2.0酶联免疫吸附测定试验系统（ELISA 2）进行了比较。ELISA 3与ELISA 2的不同之处在于，除了源自丙型肝炎病毒基因组NS3、NS4和核心区的重组抗原外，它还纳入了丙型肝炎病毒重组抗原NS5。检测的样本包括从21例输血后感染丙型肝炎病毒并正在发生血清转化的个体获取的系列血液样本。ELISA 3显示出比ELISA 2显著更高的灵敏度，在24%（5/21）的病例中能更早地检测到血清转化。虽然其中1例病例似乎代表了对NS5的早期血清转化，但ELISA 3灵敏度的提高大部分似乎源于抗-c33c检测能力的增强。
