维甲酸在体外上调小鼠角质形成细胞的c-kit配体生成，并在体内增加皮肤肥大细胞。

Retinoic acid upregulates c-kit ligand production by murine keratinocyte in vitro and increases cutaneous mast cell in vivo.

作者信息

Katayama I, Otoyama K, Yokozeki H, Nishioka K

机构信息

Department of Dermatology, Tokyo Medical and Dental University School of Medicine, Japan.

出版信息

J Dermatol Sci. 1995 Jan;9(1):27-35. doi: 10.1016/0923-1811(94)00349-j.

DOI:10.1016/0923-1811(94)00349-j

PMID:7537079

Abstract

Mouse-transformed epidermal cell line (Pam 212) generated the soluble mediators for promoting the growth of a mast cell line (MC9) in the presence of retinoic acid at a concentration of 10(-6)-10(-7) M. The effective molecule of MC9 cell growth promoting factor (MC9-GF) was non-dialyzable and eluted between the molecular weight of 45 K and 68 K on a TSK 2000 G column. Chromatofocusing analysis revealed that this factor had a pI range between 7.0 and 7.5. Anti-c-kit ligand antibody abrogated MC9-GF activity and RT-PCR analysis demonstrated that retinoic acid upregulates c-kit ligand mRNA expression by Pam cells. Several recombinant cytokines including IL1-alpha, IL-1 beta, IL-2, IL-3 or IL-4 did not promote MC9 cell growth at a concentration of 100 U/ml. The presence of anti-IL-1 alpha, -IL-1 beta, -IL-2, -IL-3 or -IL-4 antibodies did not abrogate the MC9-GF activity except for anti-c-kit ligand antibody.

摘要

小鼠转化表皮细胞系（Pam 212）在浓度为10(-6)-10(-7) M的视黄酸存在下产生可溶性介质，以促进肥大细胞系（MC9）的生长。促进MC9细胞生长因子（MC9-GF）的有效分子不可透析，在TSK 2000 G柱上洗脱时分子量在45 K至68 K之间。色谱聚焦分析表明该因子的pI范围在7.0至7.5之间。抗c-kit配体抗体消除了MC9-GF活性，逆转录-聚合酶链反应（RT-PCR）分析表明视黄酸上调了Pam细胞中c-kit配体mRNA的表达。包括白细胞介素1-α（IL1-α）、白细胞介素1-β（IL-1β）、白细胞介素2（IL-2）、白细胞介素3（IL-3）或白细胞介素4（IL-4）在内的几种重组细胞因子在浓度为100 U/ml时均未促进MC9细胞生长。除抗c-kit配体抗体外，抗IL-1α、抗IL-1β、抗IL-2、抗IL-3或抗IL-4抗体的存在并未消除MC9-GF活性。