Iwabuchi T, Maruyama T, Sei Y, Adachi K
Marine Biotechnology Institute, Kamaishi Laboratories, Iwate, Japan.
J Dermatol Sci. 1995 Jan;9(1):64-9. doi: 10.1016/0923-1811(94)00351-e.
The effects of immunosuppressive peptidyl-prolyl cis-trans isomerase (PPIase) inhibitors, cyclosporin A, FK506, ascomycin and rapamycin, on hair growth initiation (anagen hair induction) in mouse were studied by topical application on the dorsal skin surface during the telogen phase of the hair cycle. Single applications of cyclosporin A and FK506 (10 to 100 nmol in 5 microliters of ethanol) induced new hair growth in 12 days within the restricted area where the compounds were applied. On the other hand, ascomycin and rapamycin did not initiate new anagen hairs even at higher doses (1 mumol in 5 to 10 microliters of ethanol). The effects of simultaneous application of the immunosuppressants were also tested by a single topical application. Ascomycin did not inhibit the anagen hair induction by cyclosporin A, but inhibited hair induction by FK506. Rapamycin inhibited new hair growth induced by cyclosporin A and FK506. These results suggest that the inhibition of PPIase is not required for the initiation of a new hair cycle in mice, and that anagen hair induction caused by cyclosporin A and FK506 is not a result of immunosuppression. The present results also indicate that a single application of an adequate quantity of cyclosporin A and FK506 is sufficient to initiate new hair growth.
通过在毛发周期的休止期将免疫抑制性肽基脯氨酰顺反异构酶（PPIase）抑制剂环孢素A、FK506、子囊霉素和雷帕霉素局部应用于小鼠背部皮肤表面，研究了它们对小鼠毛发生长起始（生长期毛发诱导）的影响。单次应用环孢素A和FK506（在5微升乙醇中含10至100纳摩尔）在涂抹化合物的受限区域内12天内诱导了新的毛发生长。另一方面，即使在更高剂量（在5至10微升乙醇中含1微摩尔）下，子囊霉素和雷帕霉素也未引发新的生长期毛发。还通过单次局部应用测试了同时应用免疫抑制剂的效果。子囊霉素不抑制环孢素A诱导的生长期毛发，但抑制FK506诱导的毛发。雷帕霉素抑制环孢素A和FK506诱导的新毛发生长。这些结果表明，抑制PPIase对于小鼠新毛发周期的起始不是必需的，并且环孢素A和FK506引起的生长期毛发诱导不是免疫抑制的结果。目前的结果还表明，单次应用适量的环孢素A和FK506足以引发新的毛发生长。
