Otto A, Lorenz G, Kopperschläger G
Institute of Biochemistry, University of Leipzig, Federal Republic of Germany.
Bioseparation. 1995 Feb;5(1):35-40.
The partitioning of streptokinase in aqueous two-phase systems, containing poly(ethylene glycol) and dextran or poly(ethylene glycol) and salt was investigated. The protein partitions in favour of the upper, PEG-rich phase, if PEG of low molecular weight and potassium phosphate or ammonium sulphate were used. This property was found to be independent of the degree of purity and was exploited for the partial purification of streptokinase from crude material. The protein was shown to exhibit negligible affinity to diverse triazine dyes applied in affinity partitioning experiments.
研究了链激酶在含有聚乙二醇和葡聚糖或聚乙二醇和盐的双水相体系中的分配情况。如果使用低分子量的聚乙二醇和磷酸钾或硫酸铵，蛋白质会分配到富含聚乙二醇的上相中。发现该性质与纯度无关，并被用于从粗原料中部分纯化链激酶。在亲和分配实验中，该蛋白质对多种三嗪染料的亲和力可忽略不计。
