血管生成在叙利亚仓鼠胰腺癌细胞系HPD-NR肿瘤生长中的作用。

The role of angiogenesis in the tumor growth of Syrian hamster pancreatic cancer cell line HPD-NR.

作者信息

Egawa S, Tsutsumi M, Konishi Y, Kobari M, Matsuno S, Nagasaki K, Futami H, Yamaguchi K

机构信息

Growth Factor Division, National Cancer Center Research Institute, Tokyo, Japan.

出版信息

Gastroenterology. 1995 May;108(5):1526-33. doi: 10.1016/0016-5085(95)90703-3.

DOI:10.1016/0016-5085(95)90703-3

PMID:7537236

Abstract

BACKGROUND/AIMS: New therapeutic approach is required for pancreatic cancer, one of the most intractable malignancies. The role of angiogenesis in the tumor growth of a Syrian hamster pancreatic cancer cell line HPD-NR, which closely resembles its human counterpart, was investigated.

METHODS

Angiogenic activity was measured as stimulation of growth of human umbilical vein endothelial cells (HUVEC), and angiogenic factors produced by HPD-NR cells were identified by reverse-transcription polymerase chain reaction and Northern blot analysis. Then in vitro and in vivo antitumor effects of a potent angiogenesis inhibitor, O-(chloroacetylcarbamoyl)fumagillol (AGM-1470), were examined.

RESULTS

The conditioned medium of HPD-NR cells stimulated the growth of HUVEC, and four hamster angiogenic factors were detected with an overexpression of transforming growth factor alpha and vascular endothelial growth factor messenger RNAs. AGM-1470 specifically inhibited the growth of HUVEC and that of HPD-NR tumors in vivo with decreased vascularity of the tumors but not the growth of HPD-NR cells in vitro.

CONCLUSIONS

The results suggest that angiogenesis plays an important role in tumor growth of HPD-NR cells and can be a new target of medical therapy for pancreatic cancer.

摘要

背景/目的：胰腺癌是最难治疗的恶性肿瘤之一，需要新的治疗方法。本研究调查了血管生成在叙利亚仓鼠胰腺癌细胞系HPD-NR肿瘤生长中的作用，该细胞系与人类胰腺癌细胞极为相似。