Zein N N, Rakela J, Persing D H
Division of Gastroenterology and Internal Medicine, Mayo Clinic Rochester, Minnesota 55905, USA.
Mayo Clin Proc. 1995 May;70(5):449-52. doi: 10.4065/70.5.449.
To evaluate the serologic reactivities in patients infected with different hepatitis C virus (HCV) genotypes to four HCV proteins that are components of the second-generation recombinant immunoblot assay.
Serum samples from 36 patients with chronic HCV infection were obtained. RNA was extracted by using chaotropic lysis and isopropanol precipitation. Reverse-transcriptase polymerase chain reaction of the NS-5 region was performed, followed by automated single-pass dideoxy sequencing of desalted amplification products. Classification of isolated HCV subtypes was based on Simmonds' system. All samples were tested for antibodies to proteins 5-1-1, C100-3, C33c, and C22-3 with the second-generation recombinant immunoblot assay.
Reactivity to protein 5-1-1 was significantly lower for patients with genotypes 2b and 3a than for those infected with HCV types 1a or 1b (P < 0.05). Antibody reactivity to the C100-3 protein was also reduced in patients infected with HCV types 2b and 3a.
These data indicate that the genotype-dependent differences in serologic reactivities are substantial among patients with chronic HCV infection.
评估感染不同丙型肝炎病毒（HCV）基因型的患者对第二代重组免疫印迹试验中四种HCV蛋白的血清学反应性。
获取36例慢性HCV感染患者的血清样本。采用离液剂裂解和异丙醇沉淀法提取RNA。进行NS-5区域的逆转录聚合酶链反应，随后对脱盐扩增产物进行自动单通道双脱氧测序。分离的HCV亚型分类基于西蒙兹系统。所有样本均采用第二代重组免疫印迹试验检测对5-1-1、C100-3、C33c和C22-3蛋白的抗体。
2b型和3a型基因型患者对5-1-1蛋白的反应性显著低于感染1a型或1b型HCV的患者（P < 0.05）。感染2b型和3a型HCV的患者对C100-3蛋白的抗体反应性也降低。
这些数据表明，慢性HCV感染患者中血清学反应性的基因型依赖性差异很大。
