Tsang C W, Leung O K, Lee P P, Pang S F
Department of Applied Biology & Chemical Technology, Hong Kong Polytechnic University, Hung Hom Kowloon.
Biochem Biophys Res Commun. 1995 Apr 26;209(3):1132-9. doi: 10.1006/bbrc.1995.1615.
The endogenous levels of 5-methoxyindole-3-acetic acid (5-MIAA) in quail pineal gland, retina and serum were determined by capillary column gas chromatography/mass spectrometry/selected ion monitoring using a deuterated internal standard and the N-pentafluoropropionyl-O-pentafluorobenzyl ester derivative. Diurnal rhythms of pineal and serum 5-MIAA with high levels at mid-light and low levels at mid-dark suggest a pineal origin of 5-MIAA in the blood. In contrast, retina displayed an opposite diurnal rhythm of 5-MIAA with high levels at night, suggesting that the biosyntheses of 5-MIAA in the quail pineal gland and retina were different. In vitro studies on incubation media indicated that both the pineal gland and retina are capable of synthesis/secretion of 5-MIAA.
使用氘代内标和N-五氟丙酰基-O-五氟苄酯衍生物，通过毛细管柱气相色谱/质谱/选择离子监测法测定鹌鹑松果体、视网膜和血清中5-甲氧基吲哚-3-乙酸（5-MIAA）的内源性水平。松果体和血清中5-MIAA的昼夜节律为光照中期水平高，黑暗中期水平低，这表明血液中5-MIAA来源于松果体。相比之下，视网膜显示出5-MIAA的昼夜节律相反，夜间水平高，这表明鹌鹑松果体和视网膜中5-MIAA的生物合成不同。对孵育培养基的体外研究表明，松果体和视网膜都能够合成/分泌5-MIAA。
