Bertini R, Luini W, Sozzani S, Bottazzi B, Ruggiero P, Boraschi D, Saggioro D, Chieco-Bianchi L, Proost P, van Damme J
Laboratories of Biotechnology, Research Center Dompé S.p.A., L'Aquila, Italy.
AIDS Res Hum Retroviruses. 1995 Jan;11(1):155-60. doi: 10.1089/aid.1995.11.155.
It is known that the HTLV-I-transformed cell line MT4 releases chemotactic activity for monocytes spontaneously. The MT4 monocyte chemoattractant was purified to homogeneity and sequencing of 25 amino acids revealed identity with the C-C chemokine macrophage inflammatory protein-1 alpha (MIP-1 alpha/LD78). An anti-MIP-1 alpha/LD78 rabbit antiserum substantially inhibited chemotaxis of the MT4 chemoattractant. MT4 cells constitutively expressed MIP-1 alpha/LD78 but not the C-C chemokines MCP-1, RANTES, and MIP-1 beta/Act2 and the C-X-C chemokines IL-8, gro alpha, and gro beta. MT4-derived MIP-1 alpha/LD78 was active on monocytes but was a weak chemoattractant for polymorphonuclear leukocytes. Thus, MIP-1 alpha/LD78 is a major monocyte chemoattractant released by HTLV-I-transformed T cells. Expression of MIP-1 alpha/LD78, a leukocyte chemotactic and myelosuppressive molecule, may play an important role in the manifestations of HTLV-I-related diseases.
已知人嗜T淋巴细胞病毒I型（HTLV-I）转化的细胞系MT4可自发释放对单核细胞具有趋化活性的物质。MT4单核细胞趋化因子被纯化至同质，对其25个氨基酸进行测序后发现与C-C趋化因子巨噬细胞炎性蛋白-1α（MIP-1α/LD78）相同。抗MIP-1α/LD78兔抗血清可显著抑制MT4趋化因子的趋化作用。MT4细胞组成性表达MIP-1α/LD78，但不表达C-C趋化因子单核细胞趋化蛋白-1（MCP-1）、调节激活正常T细胞表达和分泌因子（RANTES）以及MIP-1β/Act2，也不表达C-X-C趋化因子白细胞介素-8（IL-8）、生长调节致癌基因α（groα）和生长调节致癌基因β（groβ）。MT4来源的MIP-1α/LD78对单核细胞有活性，但对多形核白细胞是一种弱趋化因子。因此，MIP-1α/LD78是HTLV-I转化的T细胞释放的主要单核细胞趋化因子。MIP-1α/LD78作为一种白细胞趋化和骨髓抑制分子，其表达可能在HTLV-I相关疾病的表现中起重要作用。
