Akamatsu M, Hori S, Tsutsumi Y, Osamura R Y, Ohkido M
Department of Pathology, Tokai University School of Medicine, Isehara, Japan.
Pathol Int. 1995 Feb;45(2):116-22. doi: 10.1111/j.1440-1827.1995.tb03431.x.
Eosinophilic hyaline inclusions were consistently seen in the perinuclear cytoplasm of suprabasal keratinocytes in lichen amyloidosus. The inclusions, negative with amyloid staining, were immunoreactive for ubiquitin and cytokeratin, and ultrastructurally showed aggregations of fine filaments of two sizes (central thin and peripheral thick). The thin filaments were the main component in the upper epidermal layer. Four monoclonal antibodies (AE1, AE3, KL1 and CAM5.2) and one antiserum (WSS) were used for characterizing cytokeratin expression. The AE1 antibody normally stained the basal cells, but in lichen amyloidosus basal staining mostly disappeared. Instead, groups of suprabasal keratinocytes were labeled, with AE1-reactive inclusions distributed therein. In contrast, the KL1 antibody, showing suprabasal staining, failed to react with the inclusions. The inclusions were weakly reactive with the AE3 and WSS antibodies, which stained all keratinocytes. The CAM5.2 antibody was unreactive. The subepidermal amyloid deposits were negative with all the antibodies. The inclusions were ubiquitinated especially in the granular layer. Immunoelectron microscopy disclosed that ubiquitin was more densely localized in the thin filaments than in the thick ones. This indicated that cytokeratin expression and metabolism are altered in the affected epidermis, and that ubiquitin functions in the process of degradation of abnormal cytokeratin filaments.
在苔藓样淀粉样变中，嗜酸性透明包涵体始终可见于基底上层角质形成细胞的核周细胞质中。这些包涵体对淀粉样蛋白染色呈阴性，对泛素和细胞角蛋白呈免疫反应性，超微结构显示有两种大小的细丝聚集（中央细，周边粗）。细丝是表皮上层的主要成分。使用四种单克隆抗体（AE1、AE3、KL1和CAM5.2）和一种抗血清（WSS）来表征细胞角蛋白的表达。AE1抗体通常对基底细胞染色，但在苔藓样淀粉样变中，基底染色大多消失。取而代之的是，基底上层角质形成细胞群被标记，其中分布有AE1反应性包涵体。相比之下，显示基底上层染色的KL1抗体与这些包涵体无反应。这些包涵体与AE3和WSS抗体呈弱反应性，这两种抗体可对所有角质形成细胞染色。CAM5.2抗体无反应。表皮下淀粉样沉积物对所有抗体均呈阴性。这些包涵体尤其在颗粒层被泛素化。免疫电子显微镜显示，泛素在细丝中的定位比在粗丝中更密集。这表明在受影响的表皮中细胞角蛋白的表达和代谢发生了改变，并且泛素在异常细胞角蛋白丝的降解过程中起作用。
